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Related Concept Videos

Detergent Purification of Membrane Proteins01:18

Detergent Purification of Membrane Proteins

Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
Downstream Processing01:29

Downstream Processing

Downstream processing begins once fermentation is complete and involves a series of steps to recover and purify products such as acids, vitamins, antibiotics, or proteins.Cell HarvestingFor example, for intracellular protein-based products, the first step is harvesting the cells. This is typically achieved using centrifugation or filtration to separate the cells from the liquid phase.Cell Disruption for Intracellular ProductsIf the target product is intracellular, the harvested cells must be...

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Related Experiment Video

Updated: Jul 5, 2026

An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter
10:08

An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter

Published on: May 21, 2021

Overview of protein purification and characterization.

R K Scopes1

  • 1La Trobe University, Bundoora, Australia.

Current Protocols in Protein Science
|April 23, 2008
PubMed
Summary

This overview covers essential protein purification techniques, from historical context to practical lab methods and characterization. It provides researchers with a comprehensive guide for successful protein isolation and analysis.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Protein Science

Background:

  • Historical development of protein purification techniques.
  • Importance of protein purification in biological research.

Purpose of the Study:

  • To provide a comprehensive overview of protein purification.
  • To guide researchers on methods, analysis, and equipment for protein purification.

Main Methods:

  • Discussion of various protein purification procedures.
  • Methods for protein analysis and detection.
  • Techniques for characterizing purified proteins.

Main Results:

  • Detailed recommendations for purification protocols.
  • Insights into essential laboratory equipment for protein purification.

More Related Videos

High-throughput Purification of Affinity-tagged Recombinant Proteins
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High-throughput Purification of Affinity-tagged Recombinant Proteins

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Affinity Purification of a 6X-His-Tagged Protein using a Fast Protein Liquid Chromatography System
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Affinity Purification of a 6X-His-Tagged Protein using a Fast Protein Liquid Chromatography System

Published on: April 26, 2024

Related Experiment Videos

Last Updated: Jul 5, 2026

An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter
10:08

An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter

Published on: May 21, 2021

High-throughput Purification of Affinity-tagged Recombinant Proteins
07:44

High-throughput Purification of Affinity-tagged Recombinant Proteins

Published on: August 26, 2012

Affinity Purification of a 6X-His-Tagged Protein using a Fast Protein Liquid Chromatography System
07:19

Affinity Purification of a 6X-His-Tagged Protein using a Fast Protein Liquid Chromatography System

Published on: April 26, 2024

Conclusions:

  • Protein purification is a critical step in biological research.
  • This guide equips researchers with knowledge for effective protein isolation and characterization.