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Related Concept Videos

Imaging Studies II: Positron Emission Tomography and Scintigraphy01:25

Imaging Studies II: Positron Emission Tomography and Scintigraphy

Positron Emission Tomography (PET) is a medical imaging technique that provides crucial insights into the body's physiological functions at a molecular level. It is an indispensable resource for diagnosing, staging, and monitoring various illnesses, notably cancer, neurological disorders, and cardiovascular conditions.
Fundamental Principles of PET
X-ray Imaging01:24

X-ray Imaging

German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with X-rays, and by 1900, X-ray was widely...
Radiological Investigation III: Pulmonary Angiogram and PET Scan01:13

Radiological Investigation III: Pulmonary Angiogram and PET Scan

Radiological investigations are paramount in the diagnosis and management of various pulmonary diseases. Two essential investigations are the Pulmonary Angiogram and the Positron Emission Tomography (PET) Scan.
Pulmonary Angiogram
A Pulmonary Angiogram is an invasive procedure involving injecting a contrast medium through a catheter threaded into the pulmonary artery or the right side of the heart to visualize the pulmonary vasculature. Computed Tomography (CT) scans have mainly replaced this...
Imaging Studies for Cardiovascular System III: X-Ray01:20

Imaging Studies for Cardiovascular System III: X-Ray

The most common cardiovascular diagnostic test is an X-ray. It produces images of the heart, blood vessels, and adjacent structures.
Definition and Purpose
An X-ray, or radiograph, is a non-invasive method that uses ionizing radiation to take images of internal structures. It is mainly used in cardiac imaging to examine the heart, lungs, and major blood vessels, aiming to identify abnormalities in the heart's size, shape, and position, such as heart failure, congenital defects, and vascular...
Positron Emission Tomography01:29

Positron Emission Tomography

Positron emission tomography (PET) is a medical imaging technique involving radiopharmaceuticals — substances that emit short-lived radiation. Although the first PET scanner was introduced in 1961, it took 15 more years before radiopharmaceuticals were combined with the technique and revolutionized its potential.
One of the main requirements of a PET scan is a positron-emitting radioisotope, which is produced in a cyclotron and then attached to a substance used by the part of the body being...
Radiological Investigation I: X-ray and CT01:30

Radiological Investigation I: X-ray and CT

Radiological investigations, including X-rays and computed tomography (CT) scans, are critical for diagnosing and evaluating various medical conditions. These imaging techniques provide valuable insights into the body's internal structures, aiding in the detection of abnormalities, assessment of disease progression, and development of treatment strategies. This article delves into two primary radiological investigations, chest X-rays and CT scans, outlining their purpose, procedures, and the...

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Related Experiment Video

Updated: Jul 5, 2026

Autoradiography as a Simple and Powerful Method for Visualization and Characterization of Pharmacological Targets
10:16

Autoradiography as a Simple and Powerful Method for Visualization and Characterization of Pharmacological Targets

Published on: March 12, 2019

Autoradiography.

D C Bundy1

  • 1Eastman Kodak Company, New Haven, Connecticut, USA.

Current Protocols in Protein Science
|April 23, 2008
PubMed
Summary
This summary is machine-generated.

This study details autoradiography techniques for mapping radioisotope distribution in samples. It covers film handling, quantitative analysis, and optimal exposure and processing for accurate results.

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Last Updated: Jul 5, 2026

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Area of Science:

  • Biophysical techniques
  • Radiochemistry
  • Microscopy

Background:

  • Autoradiography is crucial for visualizing the spatial distribution of labeled substances.
  • Accurate application requires understanding film properties and handling procedures.
  • Quantitative analysis is essential for reliable experimental data.

Purpose of the Study:

  • To provide a comprehensive overview of autoradiography theory and application.
  • To guide users on selecting and handling appropriate films.
  • To explain quantitative autoradiography for data calibration.

Main Methods:

  • Discussion of various film types and their correct handling.
  • Description of quantitative autoradiography for experimental calibration.
  • Guidelines for film pre-flashing, exposure, and processing.

Main Results:

  • Detailed explanation of autoradiography principles and practical applications.
  • Information on optimizing film usage for radioisotope detection.
  • Methodology for calibrating results using quantitative autoradiography.

Conclusions:

  • Effective autoradiography relies on proper film selection and handling.
  • Quantitative methods enhance the accuracy and reliability of spatial distribution data.
  • Standardized procedures for pre-flashing, exposure, and processing are vital for reproducible results.