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Detection of phosphorylation by immunological techniques

B M Sefton1

  • 1The Salk Institute, San Diego, California, USA.

Current Protocols in Protein Science
|April 23, 2008
PubMed

Phosphorylation of unlabeled proteins can be detected using immunological or enzymatic techniques. Anti-phosphotyrosine antibodies are used with immunoblots to detect tyrosine phosphorylation. This unit presents a protocol employing anti-phosphotyrosine antibodies with detection by either (125)I-labeled protein A or enhanced chemiluminescence (ECL).

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