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An Integrated Approach for Microprotein Identification and Sequence Analysis

An Integrated Approach for Microprotein Identification and Sequence Analysis

Published on: July 12, 2022

Searching sequence databases over the internet: protein identification using MS-tag.

C R Jiménez¹, L Huang, Y Qiu

¹University of California, San Francisco, San Francisco, California, USA.

Current Protocols in Protein Science

|April 23, 2008

Summary

This summary is machine-generated.

Post-source decay (PSD) analysis and tandem mass spectrometry improve protein identification when peptide mass mapping fails. These methods, along with MS-Tag software, aid in accurate protein discovery from limited peptide data.

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Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis

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Published on: May 23, 2021

Area of Science:

Proteomics
Mass Spectrometry
Bioinformatics

Background:

Peptide mass mapping is a rapid protein identification technique.
Its accuracy can be limited by sample purity, database errors, and insufficient peptide detection.

Purpose of the Study:

To address limitations in protein identification using mass spectrometry.
To describe practical aspects of obtaining and analyzing Post-Source Decay (PSD) spectra.
To introduce MS-Tag software for protein identification with limited peptide data.

Main Methods:

Post-Source Decay (PSD) analysis to generate partial amino acid sequences.
Tandem mass spectrometry for fragment ion analysis.
MS-Tag database interrogation program for matching peptide fragment ions to protein sequences.
MALDI-collision-induced dissociation (MALDI-CID) as an alternative method.

Main Results:

PSD analysis provides crucial partial sequence information for reliable protein identification.
MS-Tag facilitates protein identification even with a limited number of fragment ions.
The software supports single-mismatch-tolerant searching for enhanced accuracy.

Conclusions:

Post-source decay (PSD) analysis is essential for accurate protein identification when standard peptide mass mapping is insufficient.
MS-Tag offers a user-friendly and effective tool for protein discovery using limited peptide data.
MALDI-CID serves as a viable alternative to MALDI-PSD for protein analysis.