Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Phage-based expression cloning to identify interacting proteins.

J M Stone1

  • 1Massachusetts General Hospital, Boston, USA.

Current Protocols in Protein Science
|April 23, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Barriers to uptake of harm reduction techniques for GBMSM who use drugs in night-clubs and sex-on-premises venues in London and the Southeast: a mixed-methods, qualitative study.

Harm reduction journal·2025
Same author

Early Prophylactic Gastrectomy for the Management of Gastric Adenomatous Proximal Polyposis Syndrome (GAPPS).

ACS case reviews in surgery·2023
Same author

High-resolution air-clad imaging fibers.

Optics letters·2018
Same author

Response to initial antipsychotic treatment in first episode psychosis is related to anterior cingulate glutamate levels: a multicentre <sup>1</sup>H-MRS study (OPTiMiSE).

Molecular psychiatry·2018
Same author

Low index contrast imaging fibers.

Optics letters·2017
Same author

Endoscopic sensing of alveolar pH.

Biomedical optics express·2017
Same journal

De Novo Protein Design Using the Blueprint Builder in Rosetta.

Current protocols in protein science·2020
Same journal

Methods for Expression of Recombinant Proteins Using a Pichia pastoris Cell-Free System.

Current protocols in protein science·2020
Same journal

Histone Purification Combined with High-Resolution Mass Spectrometry to Examine Histone Post-Translational Modifications and Histone Variants in Caenorhabditis elegans.

Current protocols in protein science·2020
Same journal

A Rapid and Facile Purification Method for Glycan-Binding Proteins and Glycoproteins.

Current protocols in protein science·2020
Same journal

Synthesis of Recombinant Human Hemoglobin With NH<sub>2</sub> -Terminal Acetylation in Escherichia coli.

Current protocols in protein science·2020
Same journal

A Fluorescence-Based Assay to Monitor SUMOylation in Real-Time.

Current protocols in protein science·2020
See all related articles

Interaction cloning identifies interacting genes using a bait protein probe. This method bypasses traditional protein purification and sequencing for faster gene discovery.

Area of Science:

  • Molecular Biology
  • Genetics

Background:

  • Interaction cloning, also known as expression cloning, is a method for identifying genes encoding proteins that interact with a specific
  • bait
  • protein.

Purpose of the Study:

  • To present a detailed protocol for interaction cloning using a fusion protein probe.

Main Methods:

  • A fusion protein of bait protein and glutathione-S-transferase (GST) with a protein kinase A site is constructed.
  • This labeled fusion protein is used to probe a bacteriophage cDNA expression library.
  • Interaction is detected by screening for plaques expressing interacting proteins via a radiolabeled bait protein probe.

Main Results:

  • Direct isolation of genes encoding interacting proteins is achieved.

Related Experiment Videos

  • The technique circumvents the need for protein purification, microsequencing, or antibody production.

    • Conclusions:

    • Interaction cloning offers an efficient strategy for identifying interacting proteins and their corresponding genes.
    • This method streamlines the process of gene discovery in molecular biology research.