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Related Concept Videos

Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
Precipitation and Co-precipitation01:17

Precipitation and Co-precipitation

Precipitation and coprecipitation methods can be used to separate a mixture of ions in a solution. In qualitative inorganic analysis, ions that form sparingly soluble precipitates with the same reagent are separated based on the differences in solubility products. For example, consider the separation of Cu(II) and Fe(II) ions by precipitation as insoluble sulfides. First, copper(II) sulfide is precipitated by the addition of acidic H2S, where the dissociation of H2S is suppressed. Adding H2S...
Types Of Column Chromatography01:29

Types Of Column Chromatography

The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...
Precipitate Formation and Particle Size Control01:16

Precipitate Formation and Particle Size Control

In precipitation gravimetry, the precipitating agent should react specifically or selectively with the analyte. While a specific reagent reacts with the analyte alone, a selective reagent can react with a limited number of chemical species.
The obtained precipitate should be either a pure substance of known composition or easily converted to one by a simple process, such as ignition or drying. In addition, the precipitate should be insoluble and easily filterable. In general, filterability...
Detergent Purification of Membrane Proteins01:18

Detergent Purification of Membrane Proteins

Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
Downstream Processing01:29

Downstream Processing

Downstream processing begins once fermentation is complete and involves a series of steps to recover and purify products such as acids, vitamins, antibiotics, or proteins.Cell HarvestingFor example, for intracellular protein-based products, the first step is harvesting the cells. This is typically achieved using centrifugation or filtration to separate the cells from the liquid phase.Cell Disruption for Intracellular ProductsIf the target product is intracellular, the harvested cells must be...

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Related Experiment Video

Updated: Jul 5, 2026

Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry
11:12

Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry

Published on: February 7, 2022

Selective precipitation of proteins.

R E Lovrien1, D Matulis

  • 1University of Minnesota, St. Paul, Minnesota, USA.

Current Protocols in Protein Science
|April 23, 2008
PubMed
Summary

Selective protein precipitation is a versatile technique for protein recovery and purification from cell lysates. This guide details methods, optimization parameters, and protocols for effective protein isolation.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Protein Chemistry

Background:

  • Selective protein precipitation is a key technique in biochemistry.
  • It enables the recovery and fractionation of proteins from complex mixtures like cell lysates.
  • Different methods exist, each with specific applications in protein purification.

Purpose of the Study:

  • To describe various methods for selective protein precipitation.
  • To explain the underlying physical and chemical principles of these methods.
  • To provide guidance on optimizing precipitation protocols for protein recovery.

Main Methods:

  • Outlining multiple selective precipitation protocols.
  • Detailing the physical or chemical basis for each precipitation method.

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An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter
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An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter

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Last Updated: Jul 5, 2026

Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry
11:12

Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry

Published on: February 7, 2022

Affinity Purification of a 6X-His-Tagged Protein using a Fast Protein Liquid Chromatography System
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Affinity Purification of a 6X-His-Tagged Protein using a Fast Protein Liquid Chromatography System

Published on: April 26, 2024

An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter
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An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter

Published on: May 21, 2021

  • Describing optimization parameters and basic steps for protocol development.

    • Main Results:

    • Provides a comprehensive overview of selective protein precipitation techniques.
    • Explains how to tailor precipitation for bulk recovery, fractionation, or single-protein isolation.
    • Offers a framework for developing optimized precipitation protocols.

    Conclusions:

    • Selective precipitation is a flexible and powerful tool for protein purification.
    • Understanding the principles allows for optimization across various applications.
    • This unit serves as a practical guide for researchers.