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Related Experiment Video

Updated: Jul 5, 2026

High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension
11:42

High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension

Published on: December 28, 2015

Choice of cellular protein expression system.

D Gray1, S Subramanian

  • 1Chiron Corporation, Emeryville, California, USA.

Current Protocols in Protein Science
|April 23, 2008
PubMed
Summary
This summary is machine-generated.

Choosing the right recombinant protein expression system is crucial for successful protein production. This guide covers expression choices, post-translational modifications, folding, and downstream effects for optimal results.

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Related Experiment Videos

Last Updated: Jul 5, 2026

High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension
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Published on: December 28, 2015

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Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization
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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Recombinant protein expression is a fundamental technique in molecular biology and biotechnology.
  • A diverse array of expression systems and methodologies are currently available for protein production.

Purpose of the Study:

  • To provide an overview of critical factors influencing recombinant protein expression and production.
  • To guide researchers in selecting optimal expression strategies for specific proteins.

Main Methods:

  • Discussion of various expression systems and associated techniques.
  • Analysis of post-translational modifications (PTMs) such as glycosylation, acylation, and sulfation.
  • Consideration of in vivo and in vitro protein folding processes.
  • Evaluation of the impact of downstream elements on expression efficiency.

Main Results:

  • The selection of an appropriate expression system significantly impacts protein yield and quality.
  • Post-translational modifications are critical for protein function and require careful consideration.
  • Protein folding, whether in vivo or in vitro, is a key determinant of functional protein production.
  • Downstream elements can profoundly influence the success of recombinant protein expression.

Conclusions:

  • Optimal recombinant protein expression necessitates a careful evaluation of multiple factors.
  • Tailoring the expression strategy to the specific protein and desired modifications is essential for success.
  • Understanding PTMs, folding, and downstream influences allows for enhanced protein production.