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Related Concept Videos

Chromatography: Introduction01:10

Chromatography: Introduction

Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
Principles Of Column Chromatography01:13

Principles Of Column Chromatography

The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
Chromatographic Methods: Classification01:12

Chromatographic Methods: Classification

Chromatographic techniques are classified in three ways: the classification is based on the physical state of the stationary and mobile phases, how the mobile phase and the stationary phase contact each other, or through the chemical or physical processes that isolate the components of the sample. Typically, the mobile phase is either a liquid or gas, while the stationary phase is either a solid or a liquid layer applied to a solid surface.
Chromatographic techniques are typically named by...
Chromatographic Methods: Terminology01:18

Chromatographic Methods: Terminology

Chromatography is an analytical technique widely used in fields such as chemistry, biology, environmental science, and pharmaceuticals to separate the components of a mixture and identify substances between them. The process of chromatography is based on the interactions between two distinct phases: the stationary phase and the mobile phase. The stationary phase is fixed in place by a supporting material, while the mobile phase moves over it, carrying the solutes. As the mobile phase travels,...
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
Types Of Column Chromatography01:29

Types Of Column Chromatography

The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...

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Related Experiment Video

Updated: Jul 5, 2026

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
10:14

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Published on: September 2, 2020

Overview of conventional chromatography.

A Williams1

  • 1Pharmacia Biotech, Piscataway, New Jersey, USA.

Current Protocols in Protein Science
|April 23, 2008
PubMed
Summary

Developing a successful protein purification strategy requires a tailored approach, combining multiple separation techniques based on protein and contaminant characteristics. This overview details essential steps from source material selection to final polishing for effective protein isolation.

Area of Science:

  • Biochemistry
  • Protein Chemistry
  • Separation Science

Background:

  • Protein purification is crucial for biochemical research and therapeutic development.
  • No universal purification method exists, necessitating customized strategies.
  • Effective purification relies on understanding target protein and contaminant properties.

Purpose of the Study:

  • To outline a systematic approach for developing successful protein purification strategies.
  • To guide researchers in selecting and applying appropriate separation techniques.
  • To cover key stages from initial capture to final polishing.

Main Methods:

  • Discusses the selection of appropriate source materials.
  • Details the progression through different purification stages (capture, intermediate, polishing).

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Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
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Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

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Curtain Flow Column: Optimization of Efficiency and Sensitivity
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Curtain Flow Column: Optimization of Efficiency and Sensitivity

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Related Experiment Videos

Last Updated: Jul 5, 2026

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
10:14

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Published on: September 2, 2020

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
10:21

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

Published on: September 21, 2011

Curtain Flow Column: Optimization of Efficiency and Sensitivity
06:44

Curtain Flow Column: Optimization of Efficiency and Sensitivity

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  • Explains factors influencing chromatographic resolution.

    • Main Results:

    • Highlights the necessity of a multi-technique approach tailored to specific proteins.
    • Emphasizes the importance of understanding protein and contaminant properties for strategy design.
    • Provides a framework for optimizing purification processes.

    Conclusions:

    • A well-defined strategy combining multiple separation techniques is essential for successful protein purification.
    • The described approach, from source selection to polishing, offers a robust method for protein isolation.
    • Optimizing chromatographic resolution is a key factor in achieving high purity.