Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Hydrophobic-interaction chromatography.

¹Pharmacia Biotech, Piscataway, New Jersey, USA.

Current Protocols in Protein Science

|April 23, 2008

Summary

This summary is machine-generated.

Learn key hydrophobic interaction chromatography (HIC) parameters for effective separation. This guide covers sample preparation, matrix selection, and column optimization for reproducible results.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Tidal Energy: The benthic effects of an operational tidal stream turbine.

Marine environmental research·2017

Same author

Experimental and Monte Carlo determination of the TG-43 dosimetric parameters for the model 9011 THINSeed brachytherapy source.

Medical physics·2010

Same author

Pharmacological management of pain and anxiety during emergency procedures in children.

Paediatric drugs·2001

Same author

A randomized clinical trial of continuous-flow nitrous oxide and midazolam for sedation of young children during laceration repair.

Annals of emergency medicine·2001

Same author

Midazolam with ketamine: who benefits?

Annals of emergency medicine·2000

Same author

The "ouchless emergency department". Getting closer: advances in decreasing distress during painful procedures in the emergency department.

Pediatric clinics of North America·2000

Same journal

De Novo Protein Design Using the Blueprint Builder in Rosetta.

Current protocols in protein science·2020

Same journal

Methods for Expression of Recombinant Proteins Using a Pichia pastoris Cell-Free System.

Current protocols in protein science·2020

Same journal

Histone Purification Combined with High-Resolution Mass Spectrometry to Examine Histone Post-Translational Modifications and Histone Variants in Caenorhabditis elegans.

Current protocols in protein science·2020

Same journal

A Rapid and Facile Purification Method for Glycan-Binding Proteins and Glycoproteins.

Current protocols in protein science·2020

Same journal

Synthesis of Recombinant Human Hemoglobin With NH<sub>2</sub> -Terminal Acetylation in Escherichia coli.

Current protocols in protein science·2020

Same journal

A Fluorescence-Based Assay to Monitor SUMOylation in Real-Time.

Current protocols in protein science·2020

See all related articles

Area of Science:

Biochemistry
Analytical Chemistry
Chromatography

Background:

Hydrophobic interaction chromatography (HIC) is a powerful technique for protein purification.
Optimizing HIC requires careful consideration of multiple experimental parameters.

Purpose of the Study:

To provide a comprehensive overview of essential parameters for designing and optimizing HIC separations.
To offer practical protocols for HIC column preparation, operation, and maintenance.

Main Methods:

Discussion of critical parameters: sample preparation, matrix, column, and buffer selection.
Detailed protocols for column packing, testing, condition determination, elution, and column care.

Main Results:

Related Experiment Videos

Effective HIC separation relies on meticulous control of binding and elution conditions.

Proper column maintenance ensures reproducibility and longevity of HIC columns.

Conclusions:

This unit provides a foundational guide for successful HIC method development and application.
Implementing these protocols facilitates efficient and reliable biomolecule purification using HIC.