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Updated: Jul 5, 2026

Safety Precautions and Operating Procedures in an (A)BSL-4 Laboratory: 2. General Practices
Published on: October 3, 2016
Laboratory safety
George Lunn1, Gretchen Lawler Chemical Resistance Of Gloves2
1Baltimore, Maryland.
Abstract:
This appendix provides protocols for some commonly used disposal and decontamination procedures along with analytical techniques that are used to verify that reagents have been decontaminated. Some of the specific reagents covered are diaminobenzidine, ethidium bromide, cyanogen bromide and chloromethylsilane. With modification, these assays may also be used to determine the concentration of a particular chemical. Precautions are also detailed for routine handling of viable pathogenic microorganisms, as well as all human-derived materials, because they may harbor dangerous pathogens such as human immunodeficiency virus (HIV), hepatitis B virus (HBV), cytomegalovirus (CMV), Epstein-Barr virus (EBV), and a host of bacterial pathogens.

