Related Experiment Video
Updated: Jul 5, 2026
10:55
Staining Proteins in Gels
Published on: July 8, 2008
Protein detection in gels using fixation
Lynn A Echan1, David W Speicher1
1The Wistar Institute, Philadelphia, Pennsylvania.
Current Protocols in Protein Science
|April 23, 2008
Abstract:
This unit has been recently updated to include information on preformulated gel stains as well as new protocols for Sypro Ruby and silver staining, and gel imaging methodology. Other previously published protocols are also provided for both rapid and acid-based Coomassie blue staining, and alternate methods for silver staining (i.e., nonammoniacal silver staining, rapid silver staining, and an enhanced-background, two-stage method).
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The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
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Biological samples, such as cells...
01:27SDS-PAGE
Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
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02:35DNA Agarose Gel Electrophoresis
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