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C-terminal sequence analysis.

Tomas Bergman¹, Ella Cederlund, Hans Jörnvall

¹Karolinska Institutet, Stockholm, Sweden, USA.

Current Protocols in Protein Science

|April 23, 2008

Summary

Protein C-terminal sequence analysis confirms protein identity and processing. Automated chemical and manual enzymatic methods are described for determining this crucial sequence information.

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Area of Science:

Biochemistry
Proteomics
Molecular Biology

Background:

C-terminal sequence analysis is vital for protein characterization.
It aids in identifying protein processing, cleavage, and N-terminally blocked samples.
This information is crucial for gene cloning via oligonucleotide probe design.

Purpose of the Study:

To describe methods for determining C-terminal sequence information.
To provide options for both automated and manual analysis.
To facilitate protein research and gene cloning.

Main Methods:

Automated chemical sequencing method.
Manual enzymatic method using carboxypeptidase digestion.
Standard amino acid analysis for enzymatic method.

Main Results:

Successful application of both automated and manual methods.
Characterization of protein processing and cleavage at the C-terminus.
Generation of partial sequence data for N-terminally blocked proteins.

Conclusions:

C-terminal sequence analysis is a versatile tool in protein research.
Both described methods offer reliable approaches for sequence determination.
These techniques support protein identification and gene cloning efforts.

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