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Expression, purification, and characterization of aspartic endopeptidases: Plasmodium plasmepsins and "short"

Bret B Beyer1, Nathan E Goldfarb1, Ben M Dunn1

  • 1University of Florida, Gainesville, Florida.

Current Protocols in Protein Science
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Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside cells.