Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quantitative protein profile comparisons using the isotope-coded affinity tag method.

Eugene C Yi1, David R Goodlett1

  • 1Institute for Systems Biology, Seattle, Washington.

Current Protocols in Protein Science
|April 23, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Phosphoethanolamine modification of lipid A found in five distinct <i>Akkermansia</i> species.

Microbiology spectrum·2026
Same author

Association of Fontan Circulation With Gut Microbiome Derived Straight and Branched Short Chain Fatty Acids.

Journal of gastroenterology and hepatology·2026
Same author

Unveiling Diagnostic Biomarkers in Autism: A Comparative Proteome Analysis of <i>CNTNAP2</i> Knockout Mice and Human ASD Patients.

Biomolecules·2026
Same author

DMEM and EMEM as alternate growth media for pathogenic Leptospira.

PLoS neglected tropical diseases·2026
Same author

A Culture-Free Lipidomics-Based Screening Test for Uropathogens.

Clinical chemistry·2026
Same author

Benefits of Field Asymmetric Ion Mobility Spectrometry and Kendrick Mass Defect Plots in Lipid A Analysis.

Journal of the American Society for Mass Spectrometry·2026
Same journal

De Novo Protein Design Using the Blueprint Builder in Rosetta.

Current protocols in protein science·2020
Same journal

Methods for Expression of Recombinant Proteins Using a Pichia pastoris Cell-Free System.

Current protocols in protein science·2020
Same journal

Histone Purification Combined with High-Resolution Mass Spectrometry to Examine Histone Post-Translational Modifications and Histone Variants in Caenorhabditis elegans.

Current protocols in protein science·2020
Same journal

A Rapid and Facile Purification Method for Glycan-Binding Proteins and Glycoproteins.

Current protocols in protein science·2020
Same journal

Synthesis of Recombinant Human Hemoglobin With NH<sub>2</sub> -Terminal Acetylation in Escherichia coli.

Current protocols in protein science·2020
Same journal

A Fluorescence-Based Assay to Monitor SUMOylation in Real-Time.

Current protocols in protein science·2020
See all related articles

Quantitative protein profiling uses mass spectrometry with differential stable isotopic labeling to measure protein expression changes between biological states. The isotope-coded affinity tag (ICAT) technique enables simultaneous comparison of numerous proteins.

Area of Science:

  • Proteomics
  • Biochemistry
  • Analytical Chemistry

Background:

  • Measuring protein expression changes is crucial for understanding biological processes and disease.
  • Existing methods rely on mass spectrometry and isotopic labeling for quantitative analysis.
  • Differential labeling allows for simultaneous comparison of protein levels between samples.

Purpose of the Study:

  • To describe a popular method for quantitative protein profiling.
  • To detail the isotope-coded affinity tag (ICAT) technique for protein expression analysis.

Main Methods:

  • Utilizes differential stable isotopic labeling (in vivo or in vitro) of proteins or peptides.
  • Employs mass spectrometry for identification and quantification of labeled peptides.
  • Compares heavy-labeled and normal-isotopic peptides to determine expression changes.

Related Experiment Videos

Main Results:

  • Enables simultaneous comparison of many proteins between two biological conditions.
  • Provides quantitative data on protein expression alterations.
  • Facilitates analysis of conditions like yeast grown on different media or normal versus cancer cells.

Conclusions:

  • The described method offers a powerful approach for quantitative protein profiling.
  • The isotope-coded affinity tag (ICAT) technique is a valuable tool in proteomics research.
  • This methodology aids in comparative analysis of complex biological samples.