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Updated: Jul 5, 2026

Protease- and Acid-catalyzed Labeling Workflows Employing 18O-enriched Water

Protease- and Acid-catalyzed Labeling Workflows Employing ¹⁸O-enriched Water

Published on: February 20, 2013

Quantitative protein analysis using proteolytic [18O]water labeling.

Kristy J Reynolds¹, Catherine Fenselau¹

¹University of Maryland, College Park, Maryland.

Current Protocols in Protein Science

|April 23, 2008

Summary

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This study details a method for proteolytic oxygen-18 labeling of peptides. This technique enables accurate quantitation of relative protein levels in comparative proteomics experiments.

Area of Science:

Biochemistry
Proteomics
Analytical Chemistry

Background:

Quantitative proteomics is crucial for understanding biological processes.
Accurate measurement of relative protein levels requires robust labeling strategies.

Purpose of the Study:

To describe a procedure for proteolytic [(18)O]water labeling of peptides.
To enable quantitation of relative protein levels for comparative proteomic studies.

Main Methods:

Proteolytic digestion of proteins.
Labeling of peptides using [(18)O]water.
Mass spectrometry-based quantitation.

Main Results:

The described method allows for the labeling of peptides.

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Last Updated: Jul 5, 2026

Protease- and Acid-catalyzed Labeling Workflows Employing 18O-enriched Water

Protease- and Acid-catalyzed Labeling Workflows Employing ¹⁸O-enriched Water

Published on: February 20, 2013

Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling

Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling

Published on: July 1, 2014

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Published on: April 19, 2019

Relative protein levels can be accurately quantitated.

Conclusions:

Proteolytic [(18)O]water labeling is an effective technique for comparative proteomics.
This method facilitates the precise measurement of protein abundance changes.