Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
SDS-PAGE01:27

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

First-in-Human Study of a Novel Coronary Crossing Device for Calcified Lesions.

JACC. Cardiovascular interventions·2025
Same author

Analysis of seat belt buckle release forces in post-rollover scenarios: Implications for passenger safety.

Applied ergonomics·2025
Same author

A fatigue failure framework for the assessment of highly variable low back loading using inertial motion capture - a case study.

Ergonomics·2025
Same author

Critical review of chronic toxicity testing approaches with the saltwater mysid (Americamysis bahia) used in pesticide registration.

Environmental toxicology and chemistry·2025
Same author

Scan/rescan reliability of magnetic resonance imaging (MRI).

European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·2025
Same author

Expedited conveyance of out-of-hospital-cardiac arrest patients with STEMI and shockable rhythms to Cardiac Arrest Centres - A feasibility pilot study of the British Cardiovascular Intervention Society conveyance algorithm.

Resuscitation·2025

Related Experiment Video

Updated: Jul 5, 2026

Electroeluting DNA Fragments
06:13

Electroeluting DNA Fragments

Published on: September 5, 2010

Overview of digital electrophoresis analysis.

Scott Medberry1, Sean Gallagher2, Butch Moomaw3

  • 1Agilent Technologies, Inc, Palo Alto, California.

Current Protocols in Protein Science
|April 23, 2008
PubMed
Summary

Digital gel electrophoresis imaging offers superior quantitation and analysis over traditional photography. This method enhances biomolecule separation documentation through rapid digital capture and advanced image analysis software.

More Related Videos

Denaturing Gradient Gel Electrophoresis (DGGE)
10:52

Denaturing Gradient Gel Electrophoresis (DGGE)

Published on: February 25, 2007

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
07:58

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

Published on: September 19, 2018

Related Experiment Videos

Last Updated: Jul 5, 2026

Electroeluting DNA Fragments
06:13

Electroeluting DNA Fragments

Published on: September 5, 2010

Denaturing Gradient Gel Electrophoresis (DGGE)
10:52

Denaturing Gradient Gel Electrophoresis (DGGE)

Published on: February 25, 2007

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
07:58

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

Published on: September 19, 2018

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Biochemistry

Background:

  • Gel electrophoresis is a standard technique for separating biomolecules.
  • Traditional documentation relies on silver halide photography, suitable for qualitative analysis.
  • Digital capture presents advantages for quantitative and sophisticated gel analysis.

Purpose of the Study:

  • To highlight the benefits of digital documentation for gel electrophoresis.
  • To introduce key terms and processes in digital gel imaging.
  • To provide an overview of digital image analysis methods for gel electropherograms.

Main Methods:

  • Digital image capture using specialized devices.
  • Image manipulation and analysis using dedicated software.
  • Review of one- and two-dimensional digital image analysis techniques.

Main Results:

  • Digital imaging enables rapid data acquisition and sophisticated analysis.
  • Image analysis software facilitates accurate quantitation of biomolecules.
  • Digital methods overcome limitations of traditional photographic documentation.

Conclusions:

  • Digital gel electrophoresis imaging is crucial for quantitative biomolecule analysis.
  • Adoption of digital techniques enhances the utility and throughput of gel electrophoresis.
  • This approach provides robust and reproducible results for molecular biology research.