Enzymic thin film coatings for bioactive materials

Area of Science:

• Biomaterials Science
• Enzyme Immobilization
• Surface Chemistry

Background:

• Bioactive materials are crucial for applications like biocatalysis and biosensors.
• Developing efficient methods for creating functional bioactive surfaces is an ongoing challenge.
• Enzyme immobilization on surfaces enhances stability and reusability.

Purpose of the Study:

• To introduce a unique spin coating method for preparing bioactive materials.
• To investigate the preparation and activity of protease Subtilisin Carlsberg-coated plastic films.
• To evaluate the enzyme's efficacy in hydrolyzing chicken egg albumin (CEA) in different states.

Main Methods:

• Utilized spin coating to create thin films of Subtilisin Carlsberg protease on plastic substrates.
• Quantified enzyme loading on the coated films (approximately 13 microg/cm2).
• Assessed the enzymatic activity through the hydrolysis of chicken egg albumin (CEA).

Main Results:

• The spin coating process yielded bioactive films with retained enzyme activity.
• Enzyme coatings retained 46% of their activity for CEA hydrolysis in aqueous solutions.
• The protease thin films demonstrated significant activity against solid-state CEA.

Conclusions:

• Spin coating is an effective technique for preparing active enzyme-coated materials.
• The immobilized protease exhibits robust activity against both soluble and solid-state substrates.
• This method holds potential for developing advanced functional surfaces for biocatalytic applications.