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Related Experiment Video

Updated: Jul 5, 2026

Electroporation-Based Genetic Modification of Primary Human Pigment Epithelial Cells Using the Sleeping Beauty Transposon System

Electroporation-Based Genetic Modification of Primary Human Pigment Epithelial Cells Using the Sleeping Beauty Transposon System

Published on: February 4, 2021

Gene transduction by sonoporation.

Sho Ohta¹, Kentaro Suzuki, Yukiko Ogino

¹Department of Neurobiology and Anatomy, School of Medicine, University of Utah, Salt Lake City, Utah, USA.

Development, Growth & Differentiation

|April 24, 2008

Summary

Gene transduction using microbubbles and ultrasound (sonoporation) offers a simple method to introduce genes into mesenchymal cells. This technique is effective for studying gene functions in embryogenesis and morphogenesis with minimal tissue damage.

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Last Updated: Jul 5, 2026

Electroporation-Based Genetic Modification of Primary Human Pigment Epithelial Cells Using the Sleeping Beauty Transposon System

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Published on: February 4, 2021

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Area of Science:

Developmental Biology
Molecular Biology
Biotechnology

Background:

Gene transduction is crucial for studying gene functions and developmental processes.
Existing methods may cause significant tissue damage or have limited applicability.

Purpose of the Study:

To introduce and review sonoporation as an effective gene transduction method.
To highlight its utility in studying embryogenesis and morphogenesis.

Main Methods:

Utilizes microbubbles and ultrasound for gene delivery.
Involves simple procedures for gene transduction into mesenchymal cells.

Main Results:

Sonoporation efficiently transduces genes into mesenchymal cells.
The method causes minimal damage to target tissues.

Conclusions:

Sonoporation is a valuable and minimally invasive tool for gene transduction.
It is effective for investigating molecular mechanisms of morphogenesis and gene functions in development.