Gene transduction by sonoporation
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Updated: Jul 5, 2026
Electroporation-Based Genetic Modification of Primary Human Pigment Epithelial Cells Using the Sleeping Beauty Transposon System
Published on: February 4, 2021
Sho Ohta1, Kentaro Suzuki, Yukiko Ogino
1Department of Neurobiology and Anatomy, School of Medicine, University of Utah, Salt Lake City, Utah, USA.
Abstract:
Gene transduction technologies are essential tools for understanding of gene functions or gene cascades underlying embryogenesis. In this review, we introduce a gene transduction method using microbubble and ultrasound (hereafter referred to as sonoporation). Sonoporation is carried out with relatively simple procedures and easily transduces genes into mesenchymal cells without significant damage to target tissues. Therefore, sonoporation is effective for gene transduction to study the molecular mechanisms of morphogenesis.
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