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Updated: Jul 5, 2026
Modeling Chemotherapy Resistant Leukemia In Vitro
Published on: February 9, 2016
Reversal effect of Ganoderma lucidum polysaccharide on multidrug resistance in K562/ADM cell line
Wei-Dong Li1, Bo-di Zhang, Ran Wei
1Department of Pharmacology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100083, China. lwdpharma@126.com
Aim:
To investigate the reversal effect of Ganoderma lucidum polysaccharides (Gl-PS) on multidrug resistance (MDR) in the adriamycin (ADM)-resistant leukemic cell line K562/ADM.
Methods:
Cytotoxicity was assayed by 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide method; the ADM concentration in cells was determined by flow cytometry and confocal laser scanning microscopy techniques; the expression of P-glycoprotein was assayed by flow cytometry; and the mRNA expression levels of MDR-1 and MDR-associated protein (MRP)1 were determined by RT-PCR.
Results:
Gl-PS reversed MDR in K562/ADM cells. Gl-PS obviously reversed the resistance of K562/ADM to doxorubicin. The reversing factors of Gl-PS at 10 and 20 mg/L were 6.46 and 6.80, respectively. MDR-1 and MRP1 transcription were downregulated by 10 and 50 mg/L Gl-PS.
Conclusion:
Gl-PS can reverse the MDR by downregulating the expression of MDR-1 and MRP1 in K562/ADM cells.
