Related Experiment Video
Updated: Jul 5, 2026

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
Published on: December 15, 2017
Optimization of media composition for Nattokinase production by Bacillus subtilis using response surface methodology
V Deepak1, K Kalishwaralal, S Ramkumarpandian
1Department of Biotechnology, Kalasalingam University, Krishnankoil 626190, Viruthunagar District, Tamil Nadu, India.
Abstract:
Response surface methodology and central composite rotary design (CCRD) was employed to optimize a fermentation medium for the production of Nattokinase by Bacillus subtilis at pH 7.5. The four variables involved in this study were Glucose, Peptone, CaCl2, and MgSO4. The statistical analysis of the results showed that, in the range studied; only peptone had a significant effect on Nattokinase production. The optimized medium containing (%) Glucose: 1, Peptone: 5.5, MgSO4: 0.2 and CaCl2: 0.5 resulted in 2-fold increased level of Nattokinase (3194.25U/ml) production compared to initial level (1599.09U/ml) after 10h of fermentation. Nattokinase production was checked with fibrinolytic activity.
Related Concept Videos
Methods of Medium Optimization
Designing Growth Media for Bioreactors
Upstream Processing
Production of Antibiotics
