Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Disease outcomes with ublituximab in treatment-naïve participants: subpopulation analyses of the phase 3 ULTIMATE I and II studies in participants with relapsing multiple sclerosis.

Frontiers in immunology·2026
Same author

C1-esterase Inhibitor Treatment is Associated with Immune and Vascular Pathway Modulation in PASC with Neurological Symptoms: Longitudinal Plasma Proteomics.

Cellular and molecular neurobiology·2026
Same author

Peripheral complement C4 protein in schizophrenia: Association with gene copy number and immune cell subtypes.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Why Is MS a More Frequent Complication of EBV Infection in Females?

Immunological reviews·2026
Same author

Cytotoxic T cell recognition of α-synuclein drives pathogenic immune responses in multiple system atrophy.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Postural Orthostatic Tachycardia Syndrome, Myalgic Encephalomyelitis/Chronic Fatigue Syndrome and Long COVID as Neuroimmune Disorders.

ImmunoTargets and therapy·2026

Related Experiment Video

Updated: Jul 5, 2026

Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs
11:21

Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs

Published on: September 10, 2017

Evaluating human T cell receptor gene expression by PCR.

Emanuela Gussoni1, Michael A Panzara2, Lawrence Steinman3

  • 1Children's Hospital, Boston, Massachusetts.

Current Protocols in Immunology
|April 25, 2008
PubMed
Summary

This study details using Polymerase Chain Reaction (PCR) to analyze T cell receptor gene rearrangements in human tissues. The methods allow for characterization and quantification of these important immune system transcripts.

More Related Videos

Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR
14:14

Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR

Published on: December 6, 2014

Related Experiment Videos

Last Updated: Jul 5, 2026

Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs
11:21

Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs

Published on: September 10, 2017

Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR
14:14

Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR

Published on: December 6, 2014

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • T cell receptor (TCR) gene rearrangements are crucial for adaptive immunity.
  • Characterizing TCR diversity in various human tissues is essential for understanding immune responses.
  • Previous methods for analyzing TCR transcripts have limitations in scope and detail.

Purpose of the Study:

  • To describe a Polymerase Chain Reaction (PCR) based strategy for characterizing and quantifying rearranged T cell receptor variable gene family transcripts.
  • To provide protocols for analyzing TCR transcripts in diverse human tissues and peripheral blood lymphocytes.
  • To enable the study of junctional diversity within expressed TCR genes.

Main Methods:

  • Utilizing PCR to amplify rearranged transcripts from specific T cell receptor variable gene families.
  • Applying the strategy to various human tissue sources, including brain, spinal cord, and skeletal muscle.
  • Employing a support protocol for reverse transcribing total RNA into complementary DNA (cDNA).

Main Results:

  • The described PCR strategy successfully characterizes and quantifies rearranged TCR transcripts.
  • The protocol is adaptable to different human tissue types and peripheral blood lymphocytes.
  • Junctional diversity of expressed TCR genes can be studied through cloning and sequencing of PCR-amplified cDNA.

Conclusions:

  • The presented PCR-based unit provides a robust method for analyzing TCR gene rearrangements in human samples.
  • This technique facilitates detailed investigation of immune cell populations in both healthy and diseased states.
  • The protocols support comprehensive studies of immune receptor diversity and function across various tissues.