Jove
Visualize
Contact Us

Related Concept Videos

Complement System01:27

Complement System

The complement system is a group of approximately 20 plasma proteins that strengthen the body's defenses against infections through opsonization, inflammation, and cell lysis. Opsonization involves coating pathogens with complement proteins, making them more recognizable and facilitating phagocyte engulfment. Certain complement proteins induce inflammation that attracts immune cells to the site of infection. Cell lysis involves the destruction of pathogens through the formation of a membrane...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Coagulopathies in orthopaedics: links to inflammation and the potential of individualizing treatment strategies.

Journal of orthopaedic trauma·2012
Same author

The α₂β₁ integrin is a metastasis suppressor in mouse models and human cancer.

The Journal of clinical investigation·2010
Same author

Isolation and purification of C3 from human plasma.

Current protocols in immunology·2008
Same author

Oral beta-glucan adjuvant therapy converts nonprotective Th2 response to protective Th1 cell-mediated immune response in mammary tumor-bearing mice.

Folia histochemica et cytobiologica·2007
Same author

C5a-mediated leukotriene B4-amplified neutrophil chemotaxis is essential in tumor immunotherapy facilitated by anti-tumor monoclonal antibody and beta-glucan.

Journal of immunology (Baltimore, Md. : 1950)·2005
Same author

Mechanism by which orally administered beta-1,3-glucans enhance the tumoricidal activity of antitumor monoclonal antibodies in murine tumor models.

Journal of immunology (Baltimore, Md. : 1950)·2004
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 5, 2026

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells
06:29

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

Published on: January 29, 2014

Assays for membrane complement receptors.

Lynda D O'Rear1, Gordon D Ross1

  • 1University of Louisville, Louisville, Kentucky.

Current Protocols in Immunology
|April 25, 2008
PubMed
Summary
This summary is machine-generated.

This study details methods for assaying complement receptors (CR1, CR2, CR3, CR4) on leukocytes. These assays are adaptable for human and rodent cells, aiding complement system research.

More Related Videos

Measuring Erythrocyte Complement Receptor 1 Using Flow Cytometry
07:20

Measuring Erythrocyte Complement Receptor 1 Using Flow Cytometry

Published on: May 19, 2020

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment
07:26

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment

Published on: July 18, 2017

Related Experiment Videos

Last Updated: Jul 5, 2026

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells
06:29

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

Published on: January 29, 2014

Measuring Erythrocyte Complement Receptor 1 Using Flow Cytometry
07:20

Measuring Erythrocyte Complement Receptor 1 Using Flow Cytometry

Published on: May 19, 2020

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment
07:26

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment

Published on: July 18, 2017

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Leukocytes possess receptors for complement (C) system activation products.
  • Specific receptors include those for fixed C3 fragments: CR1, CR2, CR3, and CR4.
  • Understanding these receptors is crucial for immune response studies.

Purpose of the Study:

  • To describe reliable assays for detecting complement receptors CR1, CR2, CR3, and CR4.
  • To provide protocols adaptable for both human and rodent leukocyte samples.
  • To facilitate research on leukocyte-complement interactions.

Main Methods:

  • Rosette assay protocols for adherent and nonadherent cells.
  • Detailed methods for forming and quantifying sheep erythrocyte-C3 complexes.
  • Adaptable protocols for human and rodent (mouse, rat, guinea pig) cells.

Main Results:

  • Established two basic rosette assay protocols for CR1, CR2, CR3, and CR4 detection.
  • Included three support protocols for reagent preparation (C3 complexes).
  • Demonstrated adaptability of assays for various leukocyte types and species.

Conclusions:

  • The described assays provide a robust method for quantifying complement receptors on leukocytes.
  • These protocols are versatile and can be applied across different cell types and species.
  • The methods support further investigation into the role of complement receptors in immunity.