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Related Experiment Video

Updated: Jul 5, 2026

A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model

A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model

Published on: October 30, 2019

Collagen-induced arthritis.

Edward F Rosloniec¹, Michael Cremer¹, Andrew Kang¹

¹Veterans Affairs Medical Center and University of Tennessee, Memphis, Tennessee.

Current Protocols in Immunology

|April 25, 2008

Summary

This summary is machine-generated.

Collagen-induced arthritis (CIA) is a rodent model mimicking rheumatoid arthritis. Protocols detail inducing CIA in mice and rats, purifying type II collagen (CII), and assessing immune responses.

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Last Updated: Jul 5, 2026

A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model

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Murine Model of Epicutaneously-Induced Immunomodulation

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Published on: June 24, 2025

Area of Science:

Immunology
Rheumatology
Animal Models

Background:

Collagen-induced arthritis (CIA) is a key experimental autoimmune disease model.
It shares similarities with human rheumatoid arthritis, making it valuable for research.
CIA is induced by immunizing susceptible animals with type II collagen (CII).

Purpose of the Study:

To provide detailed protocols for establishing and utilizing the CIA model in rodents.
To outline methods for purifying type II collagen (CII) and its fragments.
To describe techniques for evaluating inflammatory and immune responses in CIA.

Main Methods:

Induction of CIA in mouse and rat models via immunization with type II collagen (CII).
Purification of native CII from bovine and chicken sources.
Purification of collagen a1(II) chains and CNBr-cleaved fragments.
Assessment of joint inflammation and measurement of CII-specific B and T cell responses.

Main Results:

Established protocols for reproducible CIA induction in mice and rats.
Successful purification of CII and its fragments for experimental use.
Methods for quantifying disease severity and immune cell activity are detailed.

Conclusions:

The provided protocols enable robust research into autoimmune arthritis using the CIA model.
Detailed methods for CII purification address challenges with commercial availability.
Comprehensive assessment strategies support in-depth analysis of CIA pathogenesis and therapeutic interventions.