Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Giardiasis01:12

Giardiasis

Giardiasis is a globally prevalent intestinal infection caused by the protozoan parasite Giardia duodenalis (also known as G. lamblia or G. intestinalis). This flagellated protozoan is the most frequently identified intestinal parasite in the United States and worldwide. Transmission primarily occurs via the fecal-oral route, with infection arising from ingestion of water or food contaminated with cysts. Individuals in low-resource settings, international travelers, outdoor enthusiasts, daycare...
Amebiasis01:28

Amebiasis

Entamoeba histolytica, a protozoan parasite, is responsible for intestinal and extraintestinal amebiasis. Though a significant proportion of infections remain asymptomatic, approximately 50 million individuals annually are estimated to present with clinical disease, resulting in up to 100,000 deaths globally. The disease burden is disproportionately high in regions with lower socioeconomic status, such as parts of India, Africa, Mexico, and Latin America.Etiology and TransmissionThe infective...
American Trypanosomiasis01:22

American Trypanosomiasis

Chagas disease, or American trypanosomiasis, is a vector-borne parasitic infection caused by Trypanosoma cruzi, a flagellated protozoan (kinetoplastid) of the family Trypanosomatidae. The disease is endemic in Latin America, although cases are increasingly reported worldwide due to human migration. Transmission most commonly occurs when feces of infected triatomine bugs contaminate bite wounds or mucosal surfaces; additional routes include congenital, transfusional, transplant-related, and oral...
Leishmaniasis01:30

Leishmaniasis

Leishmaniasis is a protozoal disease caused by species of the genus Leishmania and transmitted through the bite of infected female sandflies. The parasite exists in two principal morphological forms during its life cycle. A sandfly acquires intracellular amastigotes from an infected reservoir host, such as a dog. Within the sandfly, these forms differentiate into motile, flagellated promastigotes. During a subsequent blood meal, promastigotes are injected into the human host, where they...
Symbiosis00:58

Symbiosis

Symbiotic relationships are long-term, close interactions between individuals of different species that affect the distribution and abundance of those species. When a relationship is beneficial to both species, this is called mutualism. When the relationship is beneficial to one species but neither beneficial nor harmful to the other species, this is called commensalism. When one organism is harmed to benefit another, the relationship is known as parasitism. These types of relationships often...
Toxoplasmosis01:28

Toxoplasmosis

Toxoplasmosis, a zoonotic disease caused by the protozoan Toxoplasma gondii, poses significant public health challenges globally due to its high seroprevalence and varied clinical manifestations. As an obligate intracellular parasite, T. gondii can infect all warm-blooded vertebrates, but felids are its only definitive hosts, shedding unsporulated oocysts into the environment. Humans typically acquire the infection through ingestion of tissue cysts in undercooked meat or oocysts from...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Urinary incontinence and female genital lichen sclerosus.

Journal of the European Academy of Dermatology and Venereology : JEADV·2025
Same author

Randomised Badger Culling Trial lacks evidence for proactive badger culling effect on tuberculosis in cattle: comment on Mills et al. 2024, Parts I & II.

bioRxiv : the preprint server for biology·2024
Same author

Post-acute phase and sequelae management of epidermal necrolysis: an international, multidisciplinary DELPHI-based consensus.

Orphanet journal of rare diseases·2023
Same author

Laser therapy for genital lichen sclerosus: A systematic review of the current evidence base.

Skin health and disease·2022
Same author

2021 European guideline for the management of vulval conditions.

Journal of the European Academy of Dermatology and Venereology : JEADV·2022
Same author

Vulval acne: a case series describing clinical features and management.

Clinical and experimental dermatology·2020
Same journal

A Comprehensive Experimental Guide to Studying Cross-Presentation in Dendritic Cells In Vitro.

Current protocols in immunology·2020
Same journal

Immunologic Studies in Humans.

Current protocols in immunology·2020
Same journal

Immunologic Studies in Humans.

Current protocols in immunology·2020
Same journal

Immunologic Studies in Humans.

Current protocols in immunology·2020
Same journal

Monoclonal Antibodies to Human Cell Surface Antigens.

Current protocols in immunology·2020
Same journal

Protocols for Experimental Sjögren's Syndrome.

Current protocols in immunology·2020
See all related articles

Related Experiment Video

Updated: Jul 5, 2026

Mass Isolation and In Vitro Cultivation of Intramolluscan Stages of the Human Blood Fluke Schistosoma Mansoni
12:05

Mass Isolation and In Vitro Cultivation of Intramolluscan Stages of the Human Blood Fluke Schistosoma Mansoni

Published on: January 14, 2018

Schistosomiasis.

F Lewis1

  • 1Biomedical Research Institute, Rockville, Maryland, USA.

Current Protocols in Immunology
|April 25, 2008
PubMed
Summary
This summary is machine-generated.

This study details laboratory maintenance and collection methods for Schistosoma mansoni, a medically important trematode parasite. Proper techniques for parasite stages and snail hosts are crucial for schistosomiasis research and vaccine development.

More Related Videos

Cercarial Transformation and in vitro Cultivation of Schistosoma mansoni Schistosomules
05:30

Cercarial Transformation and in vitro Cultivation of Schistosoma mansoni Schistosomules

Published on: August 16, 2011

Using Eggs from Schistosoma mansoni as an In vivo Model of Helminth-induced Lung Inflammation
09:58

Using Eggs from Schistosoma mansoni as an In vivo Model of Helminth-induced Lung Inflammation

Published on: June 5, 2012

Related Experiment Videos

Last Updated: Jul 5, 2026

Mass Isolation and In Vitro Cultivation of Intramolluscan Stages of the Human Blood Fluke Schistosoma Mansoni
12:05

Mass Isolation and In Vitro Cultivation of Intramolluscan Stages of the Human Blood Fluke Schistosoma Mansoni

Published on: January 14, 2018

Cercarial Transformation and in vitro Cultivation of Schistosoma mansoni Schistosomules
05:30

Cercarial Transformation and in vitro Cultivation of Schistosoma mansoni Schistosomules

Published on: August 16, 2011

Using Eggs from Schistosoma mansoni as an In vivo Model of Helminth-induced Lung Inflammation
09:58

Using Eggs from Schistosoma mansoni as an In vivo Model of Helminth-induced Lung Inflammation

Published on: June 5, 2012

Area of Science:

  • Parasitology
  • Immunology
  • Infectious Diseases

Background:

  • Schistosoma mansoni is a medically significant trematode parasite.
  • Schistosomiasis research involves vaccine development and immunopathology studies.
  • Accurate parasite and host maintenance is vital for experimental reproducibility.

Purpose of the Study:

  • To describe laboratory maintenance and collection procedures for Schistosoma mansoni.
  • To provide essential techniques for various parasite life stages.
  • To guide researchers in maintaining the snail intermediate host and parasite.

Main Methods:

  • Infection of mice with cercariae.
  • Collection of cercariae, schistosomules (in vitro and in vivo), adult worms, and eggs.
  • Preparation of soluble egg antigen (SEA).
  • Maintenance of the snail intermediate host and infection with miracidia.

Main Results:

  • Established protocols for obtaining different life stages of Schistosoma mansoni.
  • Detailed methods for preparing key antigenic preparations like SEA.
  • Guidance on snail host management and parasite infection.

Conclusions:

  • Standardized procedures are essential for reliable Schistosoma mansoni research.
  • Proper maintenance of both parasite and snail host prevents experimental errors.
  • These methods support ongoing immunology and vaccine development studies for schistosomiasis.