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Updated: Jul 5, 2026

In Vitro Assay of Plasmodium-Infected Red Blood Cell Killing by Cytotoxic Lymphocytes
Published on: August 17, 2022
Experimental asexual blood stage malaria immunity
1The Queensland Institute of Medical Research, Queensland, Australia.
Abstract:
This unit describes three methods for investigating the immune response to murine malaria parasites. Immunization protocols using recombinant fragments of the merozoite surface protein-1 of Plasmodium yoelii, whole blood stage malaria parasites, and live infection with parasitized red blood cells from a Plasmodium-infected donor are provided. Methods for chemotherapeutic drug care of Plasmodium-infected mice and for inducing malaria by adoptive transfer of antigen-specific T cells are included. Finally, support protocols describe methods for growing, maintaining, and cryopreserving murine asexual blood stage malaria parasites and for preparing blood stage antigen for use in ELISAs.
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