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Purification of human IgA

Todd D Pack1

  • 1Applied Biotech, San Diego, California.

Current Protocols in Immunology
|April 25, 2008
PubMed

This unit describes the purification of human immunoglobulin A (IgA).The main method utilizes an IgA-binding protein (IgA-bp) as an affinity reagent, similar to the IgG-binding proteins, protein A and protein G. An alternate protocol describes a method for isolating IgA1 using affinity column chromatography with Jacalin, an IgA1-specific lectin. Another alternate protocol describes IgA purification by conventional fractionation using size-exclusion chromatography.

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