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Hybridoma Technology

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...

Antibody Structure

Antibody Structure

Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...

Immunoprecipitation

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

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Related Experiment Video

Updated: Jul 5, 2026

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Published on: January 2, 2017

Production of polyclonal antisera.

Helen M Cooper¹, Yvonne Paterson²

¹Ludwig Institute for Cancer Research, Melbourne, Australia.

Current Protocols in Immunology

|April 25, 2008

Summary

Polyclonal antibody production offers a faster, simpler alternative to monoclonal antibodies for various research applications. These antisera are valuable for immunoprecipitation, immunoblotting, and enzyme-linked immunosorbent assays (ELISAs).

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Last Updated: Jul 5, 2026

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

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Published on: January 2, 2017

Generation of Monoclonal Antibodies Against Natural Products

Generation of Monoclonal Antibodies Against Natural Products

Published on: April 6, 2019

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

Published on: February 6, 2018

Area of Science:

Immunology
Biotechnology

Background:

Monoclonal antibody techniques offer high specificity and uniformity.
Polyclonal antibodies remain advantageous in specific research contexts.
Current methods for polyclonal antibody production can be time-consuming and resource-intensive.

Purpose of the Study:

To describe efficient protocols for producing polyclonal antisera against protein antigens.
To provide methods applicable across multiple common laboratory animal models.
To detail serum preparation from blood samples.

Main Methods:

Detailed Basic and Alternate Protocols for polyclonal antisera production in rabbits, rats, mice, and hamsters.
A Support Protocol for effective serum preparation from animal blood.
Utilizes standard laboratory equipment for accessibility.

Main Results:

Successful generation of specific polyclonal antisera in various animal models.
Demonstrated utility of produced antisera in immunoprecipitation, immunoblotting, and ELISAs.
Established protocols are less time-consuming and require less effort than monoclonal antibody production.

Conclusions:

Polyclonal antibody production is a practical and efficient method for generating specific antibodies.
These protocols provide a valuable resource for researchers needing polyclonal reagents for common immunoassays.
The described methods offer a viable alternative to monoclonal antibodies for many applications.