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Mouse Models of Cancer Study

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

Mouse Models of Cancer Study

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

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Related Experiment Video

Updated: Jul 5, 2026

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells

Published on: May 6, 2018

Mouse SaI sarcoma tumor model.

S Ostrand-Rosenberg¹

¹University of Maryland Baltimore County, Baltimore, Maryland, USA.

Current Protocols in Immunology

|April 25, 2008

Summary

This study details the establishment and methods for obtaining SaI fibrosarcoma tumor cells in mice. Researchers can use these protocols for in vivo cancer research involving solid and ascites tumor models.

Area of Science:

Oncology
Immunology
Transplantation Biology

Background:

The SaI fibrosarcoma is a transplantable tumor model derived from A/J mice.
This model can produce solid tumors via various inoculation routes (subcutaneous, intradermal, intramuscular) and ascites tumors via intraperitoneal inoculation.

Purpose of the Study:

To describe the establishment of primary solid SaI/N tumors in syngeneic mice.
To detail the methods for establishing and maintaining SaI ascites tumors.
To outline procedures for sampling in vivo-passaged SaI ascites tumor cells.

Main Methods:

Subcutaneous, intradermal, intramuscular, and intraperitoneal inoculation of SaI fibrosarcoma cells into syngeneic mice.
Establishment and serial passaging of solid and ascites tumor models.

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Surgical removal of tumors for cell sampling.

Main Results:

Successful establishment of solid SaI/N fibrosarcoma tumors.
Successful induction and maintenance of SaI ascites tumors.
Protocols for obtaining viable tumor cells for further analysis.

Conclusions:

The described methods provide reliable protocols for generating and utilizing SaI fibrosarcoma models in vivo.
These established tumor models are suitable for various research applications in cancer biology and immunology.
The procedures facilitate the collection of tumor cells for downstream experimental analyses.