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Updated: Jul 5, 2026

Isolation of Dendritic Cells from the Human Female Reproductive Tract for Phenotypical and Functional Studies
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Isolation of Dendritic Cells from the Human Female Reproductive Tract for Phenotypical and Functional Studies

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Isolation of dendritic cells.

Kayo Inaba1, William J Swiggard2, Ralph M Steinman2

  • 1Kyoto University, Kyoto, Japan.

Current Protocols in Immunology
|April 25, 2008
PubMed
Summary
This summary is machine-generated.

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This study details two methods for preparing dendritic cells (DCs), crucial antigen-presenting cells (APCs). One method isolates DCs from mouse spleen, while another generates them from bone marrow progenitor cells using GM-CSF.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Dendritic cells (DCs) are specialized antigen-presenting cells (APCs) vital for immune responses.
  • Efficient preparation of functional DCs is essential for immunological research and therapeutic applications.

Purpose of the Study:

  • To present two distinct methods for the preparation of dendritic cells (DCs).
  • To optimize the yield and purity of DCs for experimental use.

Main Methods:

  • Isolation of DCs from mouse spleen, with a collagenase digestion protocol to enhance yield.
  • Generation of DCs from mouse bone marrow progenitor cells cultured with granulocyte/macrophage colony-stimulating factor (GM-CSF).

Main Results:

  • The spleen isolation method yields a population highly enriched in accessory cell and APC function.

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Last Updated: Jul 5, 2026

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  • The bone marrow culture method generates 5-10 x 10^6 cells, with 60% expressing DC surface markers like B-7-2/CD86.

    • Conclusions:

    • Two robust methods for DC preparation are described, catering to different research needs.
    • These protocols facilitate the study and application of dendritic cells in various immunological contexts.