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Updated: Jul 5, 2026

Basophil Activation Test for Allergy Diagnosis

Basophil Activation Test for Allergy Diagnosis

Published on: May 31, 2021

Isolation of human basophils.

¹National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, USA.

Current Protocols in Immunology

|April 25, 2008

Summary

Human basophils, rare leukocytes, are challenging to isolate. This method quickly purifies basophil-enriched cell preparations, minimizing cell stimulation and histamine release for research applications.

Area of Science:

Hematology
Immunology
Cell Biology

Background:

Human basophils are the least abundant leukocytes in circulation.
Isolating pure basophil populations is technically challenging.
Basophil activation and histamine release can be artifacts of purification.

Purpose of the Study:

To develop a rapid method for isolating human basophils.
To obtain enriched basophil preparations with minimal cell activation.
To provide a reliable technique for basophil research.

Main Methods:

Isolation of basophils from peripheral blood leukocyte populations.
Utilizing a technique that minimizes cell stimulation.
Achieving preparations with 5-50% basophils.

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Main Results:

Successful isolation of basophil-enriched cell populations.
Preparations contained basophils alongside lymphocytes, neutrophils, and monocytes.
The method is rapid and reduces basophil degranulation.

Conclusions:

This technique offers a practical approach to obtaining human basophils for study.
The method's speed and low stimulation profile are advantageous.
Enriched basophil preparations facilitate research into basophil function.