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Related Experiment Video

Updated: Jul 5, 2026

The bm12 Inducible Model of Systemic Lupus Erythematosus (SLE) in C57BL/6 Mice
12:04

The bm12 Inducible Model of Systemic Lupus Erythematosus (SLE) in C57BL/6 Mice

Published on: November 1, 2015

Animal models for SLE.

Philip L Cohen1, Michael A Maldonado

  • 1University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA.

Current Protocols in Immunology
|April 25, 2008
PubMed
Summary
This summary is machine-generated.

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This study details methods for quantifying autoantibodies associated with systemic lupus erythematosus (SLE) in mice. These protocols aid in understanding SLE pathogenesis and developing targeted therapies.

Area of Science:

  • Immunology
  • Autoimmunity
  • Molecular Biology

Background:

  • Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by widespread inflammation and autoantibodies against self-antigens, primarily nuclear components.
  • Autoantibodies are implicated in causing tissue damage, with cell-mediated immunity also playing a role.

Purpose of the Study:

  • To provide standardized protocols for quantifying SLE-associated autoantibodies in mouse serum.
  • To support research into the mechanisms of SLE pathogenesis and autoimmune disease development.

Main Methods:

  • Quantification of anti-chromatin, anti-single-stranded DNA (ssDNA), and anti-double-stranded DNA (dsDNA) antibodies using ELISA.
  • Detection of rheumatoid factor.
  • Preparation protocols for chicken chromatin and dsDNA.

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Modeling Multiple Sclerosis in the Two Sexes: MOG35-55-Induced Experimental Autoimmune Encephalomyelitis
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Modeling Multiple Sclerosis in the Two Sexes: MOG35-55-Induced Experimental Autoimmune Encephalomyelitis

Published on: October 13, 2023

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Last Updated: Jul 5, 2026

The bm12 Inducible Model of Systemic Lupus Erythematosus (SLE) in C57BL/6 Mice
12:04

The bm12 Inducible Model of Systemic Lupus Erythematosus (SLE) in C57BL/6 Mice

Published on: November 1, 2015

Modeling Multiple Sclerosis in the Two Sexes: MOG35-55-Induced Experimental Autoimmune Encephalomyelitis
05:44

Modeling Multiple Sclerosis in the Two Sexes: MOG35-55-Induced Experimental Autoimmune Encephalomyelitis

Published on: October 13, 2023

  • ELISPOT assay for enumerating autoantibody-producing cells.
  • Genotyping for faslpr and fasLgld mutations in mice.

    • Main Results:

    • Established methods for detecting and quantifying key autoantibodies in murine models of SLE.
    • Included protocols for preparing essential reagents and genetic analysis of disease-associated mutations.
    • Developed an immunoglobulin allotype-specific ELISA for chimeric animal studies.

    Conclusions:

    • The described protocols offer a comprehensive toolkit for researchers studying SLE and related autoimmune conditions in mouse models.
    • These methods facilitate the investigation of autoantibody production and cellular immunity in SLE pathogenesis.
    • Standardized quantification aids in evaluating therapeutic interventions and understanding disease mechanisms.