Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Glioblastoma-derived extracellular vesicles released after radiation promote cognitive impairment through NFκB-mediated microglial activation.

bioRxiv : the preprint server for biology·2026
Same author

Correction: Kandimalla et al. Targeted Oral Delivery of Paclitaxel Using Colostrum-Derived Exosomes. <i>Cancers</i> 2021, <i>13</i>, 3700.

Cancers·2025
Same author

Extracellular vesicles released by ALL patients contain HNE-adducted proteins: Implications of collateral damage.

Free radical biology & medicine·2024
Same author

Role of the splenic microenvironment in chronic lymphocytic leukemia development in Eµ-TCL1 transgenic mice.

Leukemia & lymphoma·2022
Same author

Extracellular vesicles released after cranial radiation: An insight into an early mechanism of brain injury.

Brain research·2022
Same author

Inhibition of Bruton Tyrosine Kinase Reduces Neuroimmune Cascade and Promotes Recovery after Spinal Cord Injury.

International journal of molecular sciences·2022

Related Experiment Video

Updated: Jul 5, 2026

Isolation and Activation of Murine Lymphocytes
08:08

Isolation and Activation of Murine Lymphocytes

Published on: October 30, 2016

Assays for B lymphocyte function.

Subbarao Bondada1, Darrell A Robertson

  • 1University of Kentucky, Lexington, Kentucky, USA.

Current Protocols in Immunology
|April 25, 2008
PubMed
Summary

This study details methods for stimulating B cells to produce antibodies in vitro and measuring these antibodies. Techniques include enzyme-linked immunosorbent assays (ELISA) and plaque-forming cell (PFC) assays for quantifying antibody production.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • B cells are crucial for adaptive immunity, producing antibodies to combat pathogens.
  • Understanding B cell antibody production is vital for vaccine development and treating immune disorders.

Purpose of the Study:

  • To describe protocols for in vitro induction of antibody production by B cells.
  • To present methods for measuring secreted antibodies and quantifying antibody-producing cells.

Main Methods:

  • Antigenic stimulation of B cells in vitro.
  • Measurement of secreted antibodies using enzyme-linked immunosorbent assays (ELISA).
  • Quantification of antibody-producing cells via plaque-forming cell (PFC) assays (Cunningham-Szenberg, Jerne-Nordin) and ELISPOT.

Main Results:

More Related Videos

HPLC-based Assay to Monitor Extracellular Nucleotide/Nucleoside Metabolism in Human Chronic Lymphocytic Leukemia Cells
11:29

HPLC-based Assay to Monitor Extracellular Nucleotide/Nucleoside Metabolism in Human Chronic Lymphocytic Leukemia Cells

Published on: July 20, 2016

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
09:25

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

Published on: February 16, 2017

Related Experiment Videos

Last Updated: Jul 5, 2026

Isolation and Activation of Murine Lymphocytes
08:08

Isolation and Activation of Murine Lymphocytes

Published on: October 30, 2016

HPLC-based Assay to Monitor Extracellular Nucleotide/Nucleoside Metabolism in Human Chronic Lymphocytic Leukemia Cells
11:29

HPLC-based Assay to Monitor Extracellular Nucleotide/Nucleoside Metabolism in Human Chronic Lymphocytic Leukemia Cells

Published on: July 20, 2016

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
09:25

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

Published on: February 16, 2017

  • Established generalized systems for in vitro antibody production applicable to various antigens.
  • Demonstrated the utility of ELISA for secreted antibody measurement.
  • Detailed PFC assay methodologies, including modifications for different antibody classes (IgM, IgG, IgA) and total immunoglobulin-secreting B cells.

Conclusions:

  • The described protocols provide a comprehensive framework for studying B cell antibody responses in vitro.
  • These methods facilitate the analysis of antibody production and B cell activity, crucial for immunological research.