Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 5, 2026

Isolation and Activation of Murine Lymphocytes
08:08

Isolation and Activation of Murine Lymphocytes

Published on: October 30, 2016

Solubilization of lymphocytes.

Allan M Weissman1

  • 1National Cancer Institute, Bethesda, Maryland, USA.

Current Protocols in Immunology
|April 25, 2008
PubMed
Summary
This summary is machine-generated.

Detergents are essential for isolating transmembrane proteins from cell membranes. This study details methods for solubilizing membrane proteins, preserving or disrupting protein interactions during purification.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Degradation of Botulinum Neurotoxin Light Chains Using PROTACs.

International journal of molecular sciences·2024
Same author

SARS-CoV-2 papain-like protease plays multiple roles in regulating cellular proteins in the endoplasmic reticulum.

The Journal of biological chemistry·2023
Same author

Differential sensitivity of the yeast Lon protease Pim1p to impaired mitochondrial respiration.

The Journal of biological chemistry·2023
Same author

Protein expression of the gp78 E3 ligase predicts poor breast cancer outcome based on race.

JCI insight·2022
Same author

A structurally conserved site in AUP1 binds the E2 enzyme UBE2G2 and is essential for ER-associated degradation.

PLoS biology·2021
Same author

Structural basis for DNA damage-induced phosphoregulation of MDM2 RING domain.

Nature communications·2020
Same journal

A Comprehensive Experimental Guide to Studying Cross-Presentation in Dendritic Cells In Vitro.

Current protocols in immunology·2020
Same journal

Immunologic Studies in Humans.

Current protocols in immunology·2020
Same journal

Immunologic Studies in Humans.

Current protocols in immunology·2020
Same journal

Immunologic Studies in Humans.

Current protocols in immunology·2020
Same journal

Monoclonal Antibodies to Human Cell Surface Antigens.

Current protocols in immunology·2020
Same journal

Protocols for Experimental Sjögren's Syndrome.

Current protocols in immunology·2020
See all related articles

Area of Science:

  • Biochemistry
  • Cell Biology
  • Protein Chemistry

Background:

  • Transmembrane proteins are crucial for cellular functions but are embedded within lipid bilayers.
  • Studying these proteins requires their extraction from the membrane environment.
  • Detergents are commonly used to solubilize membrane proteins.

Purpose of the Study:

  • To present various methods for the solubilization of membrane proteins.
  • To describe techniques for isolating transmembrane proteins while preserving or disrupting protein-protein interactions.
  • To provide guidance on membrane purification and protein cross-linking strategies.

Main Methods:

  • Solubilization of whole lymphocytes using specific detergent conditions.
  • Optional step for disrupting protein-protein interactions during solubilization.

More Related Videos

Murine Dermal Lymphatic Endothelial Cell Isolation
05:52

Murine Dermal Lymphatic Endothelial Cell Isolation

Published on: July 21, 2023

Related Experiment Videos

Last Updated: Jul 5, 2026

Isolation and Activation of Murine Lymphocytes
08:08

Isolation and Activation of Murine Lymphocytes

Published on: October 30, 2016

Murine Dermal Lymphatic Endothelial Cell Isolation
05:52

Murine Dermal Lymphatic Endothelial Cell Isolation

Published on: July 21, 2023

  • Membrane purification techniques.
  • Protein cross-linking for studying protein relationships.

    • Main Results:

    • Established protocols for detergent-mediated solubilization of membrane proteins.
    • Demonstrated methods to control the preservation or disruption of protein-protein interactions.
    • Outlined procedures for membrane isolation and protein cross-linking.

    Conclusions:

    • Effective detergent-based strategies are crucial for the purification and study of transmembrane proteins.
    • The presented methods allow for tailored approaches to maintain or disrupt protein interactions.
    • These techniques facilitate the investigation of membrane protein structure and function.