Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Large animal models for stem and progenitor cell analysis.

Robert E Donahue1, Ken Kuramoto1, Cynthia E Dunbar1

  • 1National Institutes of Health, Bethesda, Maryland.

Current Protocols in Immunology
|April 25, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Microglial clonal dynamics and the impact of clonal hematopoiesis in autologously transplanted rhesus macaques.

Cell reports·2026
Same author

Restricting glycine uptake with bitopertin improves erythropoiesis in preclinical models of Diamond-Blackfan anemia.

Blood. Red cells & iron·2026
Same author

Clonal Selection and Evolution after Treatment of Severe Aplastic Anemia.

NEJM evidence·2026
Same author

Clonal dynamics, tolerance, and adverse events after CD45-ADC-conditioned autologous HSPC transplantation in macaques.

Blood advances·2026
Same author

Harnessing CD5 to target chimeric antigen receptor-engineered NK cells against invasive fungal infections.

Molecular therapy : the journal of the American Society of Gene Therapy·2025
Same author

Optimization of lentiviral delivery of barcoded anti-CD20 chimeric antigen receptors into rhesus macaque and human natural killer cells.

Molecular therapy. Methods & clinical development·2025

Translating hematopoietic stem cell (HSC) research from animal models to human applications is challenging. This chapter explores large animal models, including macaques, for preclinical studies of HSC therapies.

Area of Science:

  • Hematology
  • Stem Cell Biology
  • Translational Medicine

Background:

  • Hematopoiesis and hematopoietic stem cell (HSC) research faces challenges in direct extrapolation from rodent or in vitro models to human applications.
  • Significant physiological differences exist between rodent and human hematopoiesis, limiting the predictive value of these models.
  • The absence of reliable in vitro assays for human HSCs further complicates translational research.

Purpose of the Study:

  • To highlight the critical role of large animal models in advancing preclinical translational research for genetic and cellular therapies.
  • To provide an overview of commonly utilized large animal models in hematopoiesis research, detailing their specific advantages and limitations.
  • To offer practical insights into the resources and protocols necessary for conducting high-intensity preclinical experimentation with these models.

Related Experiment Videos

Main Methods:

  • Review and comparison of established large animal models: macaques, baboons, dogs, cats, and sheep.
  • Discussion of the physiological relevance of each model to human hematopoiesis.
  • Inclusion of specific protocols for supporting macaques undergoing ablative cell and gene therapy procedures.

Main Results:

  • Identification of key advantages and limitations for each large animal model in the context of hematopoietic stem cell research.
  • Demonstration of the feasibility and requirements for maintaining large animal facilities for intensive preclinical studies.
  • Outline of essential procedures for supporting advanced cell and gene therapy investigations in large animals.

Conclusions:

  • Large animal models are indispensable for bridging the gap between basic science and clinical application in hematopoietic stem cell therapy.
  • Careful selection of appropriate large animal models, considering species-specific physiology, is crucial for successful translational research.
  • The detailed protocols presented offer a practical guide for investigators aiming to establish or enhance their capacity for preclinical hematopoietic stem cell therapy research.