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Updated: Jul 5, 2026

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay

Published on: September 14, 2014

Detection assays for HIV proteins.

Mario A Ostrowski¹, Tae-Wook Chun², Bruce Cheseboro²

¹University of Toronto, Toronto, Canada.

Current Protocols in Immunology

|April 25, 2008

Summary

This summary is machine-generated.

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This study details four methods for detecting human immunodeficiency virus (HIV) proteins. Assays include ELISA, immunoblotting, immunofluorescence, and a functional test for reverse transcriptase activity, aiding in viral production assessment.

Area of Science:

Virology
Immunology
Biochemistry

Background:

Accurate detection of human immunodeficiency virus (HIV) proteins is crucial for diagnosis and monitoring.
Existing methods require updated protocols for comprehensive analysis.

Purpose of the Study:

To describe four distinct assays for the detection of HIV proteins.
To provide methods for both qualitative and quantitative assessment of viral markers.

Main Methods:

Quantitative enzyme-linked immunosorbent assay (ELISA) for protein detection.
Quantitative immunoblotting assay for specific protein identification.
Qualitative immunofluorescence assay for protein localization.
Functional assay measuring reverse transcriptase activity to indicate viral production.

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Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses

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Related Experiment Videos

Last Updated: Jul 5, 2026

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay

Published on: September 14, 2014

Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors

Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors

Published on: April 9, 2014

Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses

Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses

Published on: May 4, 2015

Main Results:

The unit outlines four validated assays for HIV protein detection.
These methods cover a range of detection sensitivities and specificities.
The functional assay provides a direct measure of active viral replication.

Conclusions:

The described assays offer a comprehensive toolkit for HIV research and diagnostics.
These methods facilitate the assessment of viral presence and activity.
This updated unit enhances the capabilities for studying HIV infection.