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Modeling Multiple Sclerosis in the Two Sexes: MOG35-55-Induced Experimental Autoimmune Encephalomyelitis

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Experimental autoimmune encephalomyelitis in the mouse.

Stephen D Miller¹, William J Karpus¹

¹Northwestern University, Chicago, Illinois.

Current Protocols in Immunology

|April 25, 2008

Summary

This summary is machine-generated.

This study provides detailed protocols for inducing experimental autoimmune encephalomyelitis (EAE) in SJL mice using myelin proteins like proteolipid protein (PLP) and myelin basic protein (MBP). It also covers methods for protein purification and potential modifications for other mouse strains.

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Induction of Experimental Autoimmune Encephalomyelitis in Mice and Evaluation of the Disease-dependent Distribution of Immune Cells in Various Tissues

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Myelin Oligodendrocyte Glycoprotein (MOG35-55) Induced Experimental Autoimmune Encephalomyelitis (EAE) in C57BL/6 Mice

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Induction of Experimental Autoimmune Encephalomyelitis in Mice and Evaluation of the Disease-dependent Distribution of Immune Cells in Various Tissues

Induction of Experimental Autoimmune Encephalomyelitis in Mice and Evaluation of the Disease-dependent Distribution of Immune Cells in Various Tissues

Published on: May 8, 2016

Area of Science:

Neuroimmunology
Autoimmune disease modeling

Background:

Experimental autoimmune encephalomyelitis (EAE) is a widely used animal model for studying demyelinating diseases like multiple sclerosis.
Induction of EAE typically involves immunization with myelin antigens.

Purpose of the Study:

To provide comprehensive materials and methods for inducing active and adoptive EAE in SJL mice.
To detail the purification of key myelin proteins, proteolipid protein (PLP) and myelin basic protein (MBP).

Main Methods:

Active induction of EAE using intact PLP and MBP.
Adoptive transfer of T cells from EAE-induced mice.
Purification protocols for PLP and MBP.

Main Results:

Established protocols for successful EAE induction in SJL mice.
Demonstrated methods for obtaining purified PLP and MBP suitable for EAE induction.

Conclusions:

The provided protocols enable reproducible EAE induction in SJL mice.
The methods facilitate the study of autoimmune responses against myelin antigens.
Adaptability of protocols for other mouse strains may require modifications.