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A method to improve cellular content for corporal tissue engineering.

Daniel Eberli1, Ricardo Susaeta, James J Yoo

  • 1Wake Forest Institute for Regenerative Medicine and Department of Urology, Wake Forest University Health Sciences, Winston-Salem, North Carolina 27154, USA.

Tissue Engineering. Part A
|April 25, 2008
PubMed
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Dynamic cell seeding in bioreactors significantly improved cellularity in engineered penile tissues, enhancing their potential for penile reconstruction and restoring erectile function.

Area of Science:

  • Regenerative Medicine
  • Urology
  • Tissue Engineering

Background:

  • Previous attempts at engineering penile corpora using autologous cells achieved basic functions but lacked sufficient cellularity for full functionality.
  • Limited cellular content in engineered corporal tissue constructs hindered the restoration of normal erectile function.

Purpose of the Study:

  • To investigate if a dynamic cell seeding approach could enhance cellularity within engineered corporal tissue constructs.
  • To determine if improved cellularity would restore normal erectile function in penile tissue engineering.

Main Methods:

  • Corporal cells were statically or dynamically seeded onto acellular corporal tissue matrices within a bioreactor system.
  • Cell-matrix complexes were implanted subcutaneously in athymic mice for analysis of cell attachment, survival, and distribution.

Related Experiment Videos

  • Histological, biochemical, and scanning electron microscopy techniques were used for evaluation.

    • Main Results:

    • Dynamic cell seeding resulted in significantly higher cellularity (71% of normal corpora) compared to static seeding (39%).
    • Histological and molecular analyses confirmed successful cell attachment and proliferation within the matrix sinusoidal walls.
    • Electron microscopy validated the improved morphology and biochemistry of dynamically seeded tissues.

    Conclusions:

    • Dynamic cell seeding in a bioreactor system enhances cellularity and improves the morphological and biochemical properties of engineered corporal tissue.
    • This improved tissue engineering approach holds promise for future penile reconstruction and restoration of erectile function.