Related Experiment Video
Updated: Jul 5, 2026

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
Published on: March 16, 2017
Purification and functional analysis of fungal nitric oxide reductase cytochrome P450nor
1Department of Biology, University of Kentucky, Lexington, Kentucky, USA.
Abstract:
Cytochrome P450nor (P450nor) is a nitric oxide (NO) reductase involved in fungal denitrification. Denitrification is a biological process in which nitrate or nitrite is reduced to gaseous nitrogen, the reverse reaction of nitrogen fixation. It therefore plays an important role in maintaining global environmental homeostasis. The involvement of P450nor in fungal denitrification indicates that denitrification not only occurs in prokaryotic bacteria, but also in eukaryotic fungi. In addition, the reduction of NO to nitrous oxide catalyzed by P450nor has added new insight into the function of cytochrome P450s, which are usually monooxygenases. Currently, five isozymes of P450nor have been isolated from the subdivisions of eumycota, and studies on the function and structure of P450nor have provided important information for both molecular mechanisms of P450 reactions and wastewater treatment. This chapter describes the screening of NO reductase activities, cloning, purification, and functional analysis of P450nor.
More Related Videos
12:08Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry
Published on: March 18, 2012
07:38A General Method for Detecting Nitrosamide Formation in the In Vitro Metabolism of Nitrosamines by Cytochrome P450s
Published on: September 25, 2017
Related Concept Videos
Preparation of Amines: Reduction of Oximes and Nitro Compounds
Though catalytic hydrogenation can reduce nitrobenzenes, the reduction is nonselective in the presence of other functional groups. For instance, if nitrobenzene contains an aldehyde group,...
Phase I Reactions: Reductive Reactions
Inorganic Nitrogen Assimilation
Microbes and the Nitrogen Cycle
Nitric Oxide Signaling Pathway
Role of Reduced Coenzymes NADH and FADH₂