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Published on: July 6, 2016
Synthesis and DNA cleaving activity of water-soluble non-conjugated thienyl tetraynes
Kohei Torikai1, Yoichi Otsuka, Makoto Nishimura
1The Institute of Scientific and Industrial Research, Osaka University, Mihogaoka, Ibaraki, Osaka 567-0047, Japan. torikai@cbl.rcast.u-tokyo.ac.jp
Abstract:
Water-soluble non-conjugated thienyl tetraynes (3-6) were synthesized and their DNA cleaving activity was evaluated using electrophoresis, atomic force microscopy (AFM) and Escherichia coli (E. coli) transformation techniques. The amino-functionalized compound 4 was shown to possess an activity to cleave plasmid DNA by both electrophoresis and E. coli transformation techniques. AFM also showed a cleavage of the circular DNA into a linear form with a formation of burst-star-shaped architectures, which were envisaged to be cross-linked DNA oligomers.
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