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Tractable Mammalian Cell Infections with Protozoan-primed Bacteria
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Bovine mastitis associated with Prototheca blaschkeae.

Sara Marques1, Eliane Silva, Christine Kraft

  • 1Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, Largo Prof. Abel Salazar, 2, 4099-003 Porto, Portugal. saramarques@mail.icav.up.pt

Journal of Clinical Microbiology
|April 25, 2008
PubMed
Summary

Prototheca mastitis in dairy cows is increasing globally. Genetic analysis identified Prototheca zopfii var. hydrocarbonea as the primary cause in Portugal, with Prototheca blaschkeae also implicated.

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Published on: August 12, 2010

Area of Science:

  • Veterinary Medicine
  • Microbiology
  • Dairy Science

Background:

  • Bovine mastitis, a significant dairy industry disease, can be caused by the green alga Prototheca.
  • Biotype II Prototheca zopfii often leads to chronic, difficult-to-treat mastitis, reducing milk production.
  • Worldwide reports of Prototheca-induced mastitis are increasing, necessitating regional identification of causative species.

Purpose of the Study:

  • To genetically characterize Prototheca isolates from bovine mastitis cases in northwest Portugal.
  • To identify the specific Prototheca species involved in recurring mastitis outbreaks unresponsive to conventional therapy.

Main Methods:

  • Collected 41 Prototheca isolates from milk of dairy cows across 22 herds over six years.
  • Utilized PCR amplification of 18S ribosomal DNA (rDNA) for genetic analysis.
  • Performed amplified rDNA restriction analysis and phylogenetic analyses of 18S rDNA sequences.

Main Results:

  • Identified 37 isolates as Prototheca zopfii var. hydrocarbonea.
  • Identified four isolates as Prototheca blaschkeae.
  • Indicated a high prevalence of P. zopfii var. hydrocarbonea mastitis in the studied region.

Conclusions:

  • Prototheca zopfii var. hydrocarbonea is a significant cause of bovine mastitis in northwest Portugal.
  • This study provides the first evidence of Prototheca blaschkeae involvement in bovine mammary gland infections.