Related Experiment Video
Updated: Jul 5, 2026

Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants
Published on: March 29, 2018
Exposure to water fluoridation and caries increment.
A J Spencer1, J M Armfield, G D Slade
1Australian Research Centre for Population Oral Health, School of Dentistry, The University of Adelaide, South Australia. john.spencer@adelaide.edu.au
Water fluoridation significantly reduces dental caries increment in children. Higher exposure to fluoridated water led to fewer cavities, particularly in primary teeth, demonstrating its effectiveness in public health.
Area of Science:
- Public Health
- Dental Research
- Epidemiology
Background:
- Dental caries remains a significant public health concern globally.
- Water fluoridation is a widely implemented public health measure for caries prevention.
- Understanding the association between water fluoridation and caries increment is crucial for optimizing oral health strategies.
Purpose of the Study:
- To investigate the relationship between exposure to water fluoridation and the increase in dental caries among children in Queensland and South Australia.
- To compare the impact of varying fluoridation coverage levels on caries increment in different Australian states.
Main Methods:
- A cohort study design was employed, involving stratified random sampling of children from fluoridated and non-fluoridated areas in South Australia and Queensland.
- Baseline data on lifetime exposure to fluoridated water, other fluoride use, and socioeconomic status were collected via questionnaires.
- Dental examinations assessed tooth surface caries status, with follow-up data collected over three years for Net Caries Increment (NCI) analysis.
Main Results:
- Increased lifetime exposure to fluoridated water was a significant predictor of lower annualised Net Caries Increment (NCI) in the deciduous dentition for both states.
- A similar association was observed for the permanent dentition, but only in Queensland.
- These findings remained significant after controlling for age, gender, other fluoride exposures, and socioeconomic status in multiple linear regression analyses.
Conclusions:
- Water fluoridation demonstrates effectiveness in reducing caries increment, even when other fluoride sources are present.
- The caries-preventive effect of fluoridated water was most pronounced in the deciduous dentition.
- The impact of water fluoridation was stronger in areas with less diffusion of food and beverages from fluoridated to non-fluoridated regions.
More Related Videos
Related Concept Videos
Development of the Oral Microbiota
Minerals
Tooth Anatomy
The Crown, Neck, and Root
The visible part of the tooth is referred to as the crown. It's covered by enamel, the hardest substance in the human body. The crown is uniquely shaped for each type of tooth, allowing for different functions such as cutting, tearing, or grinding food.
Teeth
In the bud stage, the tooth germ (an aggregation of cells) starts to form in the developing jawbone. During the cap stage, the tooth germ differentiates into enamel organ, dental papilla, and dental sac, which will later develop into the tooth's enamel, dentin and...
Essential Minerals for Bone Health
Calcium and Phosphorus
Calcium is a critical component of bones, especially in the form of calcium phosphate and calcium carbonate. Since the body cannot make calcium, it must be obtained from the diet. However, calcium cannot be absorbed from the small intestine without...
Mutagenicity and Carcinogenicity

