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Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons
Published on: September 4, 2017
Stoned B mediates sorting of integral synaptic vesicle proteins
R Mohrmann1, H J Matthies, E Woodruff
1Department of Biological Sciences, Vanderbilt University, 1210 Medical Research Building III, , Nashville, TN 37235-1634, USA.
Neuroscience
|April 26, 2008
Summary
Stoned B protein (STNB) is crucial for synaptic vesicle recycling and neurotransmission. Progressive STNB depletion impairs vesicle fusion competence, impacting synaptic function and protein sorting.
Area of Science:
- Neuroscience
- Cell Biology
- Synaptic Plasticity
Background:
- Sustainable neurotransmission relies on a continuous supply of fusion-competent synaptic vesicles.
- Mutations in the stoned locus disrupt vesicle cycling and synaptic transmission.
- The precise function of Stoned A and B proteins in vesicle reconstitution remains unclear.
Purpose of the Study:
- To investigate the effects of progressive Stoned B protein (STNB) depletion on neuromuscular synapse release properties.
- To elucidate the role of STNB in synaptic vesicle competence and recycling.
Main Methods:
- Utilized novel synthetic stnB hypomorphic alleles in Drosophila.
- Analyzed excitatory junctional current amplitude and synaptic depression during varying stimulation frequencies.
- Assessed ultrastructural changes in vesicle pools and synaptic protein levels.
Main Results:
- Decreased STNB expression (< or =35%) reduced excitatory junctional current amplitude and slowed synaptic depression.
- Recovery from synaptic depression was decelerated, indicating delayed fusion-ready vesicle recovery.
- Synaptic levels of vesicular glutamate transporter (vGLUT) and synaptotagmin-1 were reduced, while the vesicle pool remained largely unaffected.
Conclusions:
- STNB is essential for vesicle reconstitution and regulates the fusion competence of recycled vesicles.
- STNB organizes endocytic sorting of integral synaptic vesicle proteins, impacting fusion.
- STNB plays a dual role in vesicle recycling and fusion competence for sustained neurotransmission.
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