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Flat Mount Preparation for Observation and Analysis of Zebrafish Embryo Specimens Stained by Whole Mount In situ Hybridization
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Wet mounting using iodine-glycerol provides a semi-permanent preparation for microscopic observation of faecal

Ramachandran Vignesh1, Ramalingam Sekar1, Esaki Muthu Shankar1

  • 1YRG Centre for AIDS Research and Education, VHS Campus, Rajiv Gandhi Salai, Taramani, Chennai 600 113, India.

Journal of Medical Microbiology
|April 26, 2008
PubMed
Abstract

No abstract available in PubMed .

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Fixation and Sectioning01:03

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

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