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Rotationally oscillating drill (Ros-Drill) for mouse ICSI without using mercury.

Ali Fuat Ergenc1, Ming-Wen Li, Mehmet Toner

  • 1Mechanical Engineering Department, University of Connecticut, Storrs, Connecticut 06269, USA. ergenc@engr.uconn.edu

Molecular Reproduction and Development
|April 26, 2008
PubMed
Summary

A novel mercury-free "Ros-Drill" intracytoplasmic sperm injection (ICSI) technique has been developed. This advanced assisted reproductive technology (ART) shows high success rates in mice, producing healthy offspring without hazardous mercury use.

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Area of Science:

  • Reproductive Biology
  • Assisted Reproductive Technology (ART)

Background:

  • Conventional intracytoplasmic sperm injection (ICSI) faces challenges with efficiency and deployment, particularly in mouse models.
  • Piezo-assisted ICSI, while an advancement, requires mercury, posing a significant drawback.
  • Mercury use in piezo-assisted ICSI presents a critical issue for safety and environmental concerns in assisted reproduction.

Purpose of the Study:

  • To develop and evaluate a novel, mercury-free microinjection technology for intracytoplasmic sperm injection (ICSI).
  • To address the limitations of conventional and piezo-assisted ICSI by introducing a safer and potentially more efficient alternative.

Main Methods:

  • Development of the "Ros-Drill" (rotationally oscillating drill) technique, utilizing microprocessor-controlled rotational oscillations on a spiked micropipette.

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  • Elimination of mercury and piezoelectric elements, replacing them with a novel mechanical oscillation system.
  • Testing the Ros-Drill ICSI technique in mouse oocytes, assessing survival, fertilization, and blastocyst formation rates.

    • Main Results:

    • The Ros-Drill ICSI technique demonstrated high oocyte survival rates (>70%) and fertilization rates (>80%).
    • Successful blastocyst formation was achieved in approximately 50% of survived oocytes.
    • Healthy, weaned pups were born after transferring Ros-Drill ICSI-created blastocysts into surrogate mothers, validating the technique's efficacy.

    Conclusions:

    • The Ros-Drill ICSI technique offers a mercury-free and effective alternative to existing assisted reproductive technologies.
    • This automated method requires minimal training and shows significant advantages over conventional and piezo-assisted ICSI.
    • The successful live births confirm the Ros-Drill ICSI's potential to resolve key issues in reproductive biology and ART.