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Updated: Jul 5, 2026
09:18
Mycobacterial DNA Extraction using Bead Beating in Custom Buffer Followed by NGS Workflow
Published on: June 13, 2025
Use of commercial cards for freeing DNA from mycobacterial strains
Enrico Tortoli1, Serena Baruzzo, Stefania Lauria
1Regional Reference Center for Mycobacteria, Careggi Hospital, Florence, Italy. enrico.tortoli@unifi.it
The New Microbiologica
|April 29, 2008
Abstract:
A new commercial system for rapid extraction of DNA, consisting of a card on which a drop of bacterial suspension is spotted, was evaluated with 43 mycobacterial strains. Once dried, a small disk of the seeded area was directly transferred in the amplification mixture. All the samples produced good quality amplification products which were satisfactorily sequenced.
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