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[Suppressive effect of rhIL-24 protein on the human A375 cell melanoma in nude mouse]
Xiao-Yu Zhao1, Su Yan, Mei Han
1Department of Plastic Surgery, First Affiliated Hospital of Suzhou University, Suzhou, China.
Objective:
To study the suppressive effect of purified and renaturalized rhIL-24 protein on the human A375 cell melanoma in nude mouse.
Methods:
Human A375 cells were injected into the nude mouse. After the volume of tumor attained, rhIL-24 was injected into the tumor. 2 weeks later, the tumors were resected for measurement of volume and weight, following with pathological and immunohistochemistry examination.
Results:
The volume and weight of tumors were decreased markedly after treatment of rhIL-24, when compared with those in controls. The expression of Bax gene upregulated, while Bcl-2, CD34 and VEGF gene downregulated. It indicated tumor growth inhibition and inducing of apoptosis of tumor cells.
Conclusions:
rhIL-24 has a suppressive effect on the A375 cell melanoma in nude mouse. It can also induce the A375 cell apoptosis without side effect on nude mouse.