Related Experiment Video
Updated: Jul 5, 2026

Physical Isolation of Endospores from Environmental Samples by Targeted Lysis of Vegetative Cells
Published on: January 21, 2016
[Isolation of a thermophilic bacterium and analysis of its extracellular polymeric substance]
Jing Liu1, Jun Wang, Xinyu Bai
1College of Life Sciences, Nankai University, Tianjin Key Laboratory of Microbial Functional Genomics, Ministry of Education, Tianjin 300071, China. liujing8660@mail.nankai.edu.cn
Abstract:
A thermophilic bacterial strain MS-1 was isolated from the water let from an oil well, and identified as Bacillus sp. by its 16S rDNA sequence and morphological characteristics. The bacterium was able to grow at 60 degrees C and produce extracellular polymeric substance (EPS). Analysis of the EPS showed that there was 48.3% - 54.5% polysaccharide, which was composed of mannose:glucose:galactose at a ratio of 2.04 : 1.00 : 0.89. The protein fraction was 37.2% - 42.4% in EPS, containing methionine, leucine, aspartic acid, alanine, histidine and serine.Transmission Electron Microscopy and Environmental Scanning Electron Microscopy were used to observe the extracellular polymeric substances. The strain is useful in microbial profile modification in high temperature oil fields.
Related Concept Videos
Hyperthermophilic Bacteria
Techniques for Isolation of Pure Cultures
Diversity of Archaea IV
Diversity of Archaea I

