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Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

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Expression patterns of protein kinase D 3 during mouse development.

Kornelia Ellwanger¹, Klaus Pfizenmaier, Sylke Lutz

¹Institute of Cell Biology and Immunology, University of Stuttgart, Allmandring 31, 70569 Stuttgart, Germany. kornelia.ellwanger@izi.uni-stuttgart.de

BMC Developmental Biology

|April 29, 2008

Summary

Protein kinase D3 (PKD3) is crucial for mouse embryonic development. This study reveals PKD3

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Area of Science:

Molecular Biology
Developmental Biology
Biochemistry

Background:

The Protein Kinase D (PKD) family includes serine/threonine kinases with diverse cellular roles.
PKD1 and PKD2 are well-studied, implicated in Golgi organization, transport, immunity, apoptosis, and proliferation.
PKD3's functions in development were previously unknown, with in vitro studies suggesting roles in TGN transport and glucose transport.

Purpose of the Study:

To investigate the physiological functions of Protein Kinase D3 (PKD3) during mouse embryonic development.
To determine the expression pattern of PKD3 in developing mouse embryos.

Main Methods:

Immunohistochemistry was used to examine PKD3 expression.
A PKD3-specific antibody was employed for detection.

Main Results:

PKD3 exhibits differential expression during mouse embryonic organogenesis.
Strong and consistent PKD3 expression was observed in the developing heart from embryonic day 10 (E10) to E16.5.
Increased PKD3 expression was detected from E12.5 onwards in neuronal tissues, connective tissues, and skeletal muscles.

Conclusions:

The findings indicate a significant role for PKD3 in the development of the heart, nervous system, and skeletal muscles.
The study supports an important function for PKD3 during embryonic development of these specific tissues.