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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jul 5, 2026

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Technical variables in high-throughput miRNA expression profiling: much work remains to be done.

Peter T Nelson1, Wang-Xia Wang, Bernard R Wilfred

  • 1Department of Pathology and Sanders-Brown Center, University of Kentucky, Lexington, KY 40536, USA. pnels2@email.uky.edu

Biochimica Et Biophysica Acta
|April 29, 2008
PubMed
Summary

Technical pitfalls in microRNA (miRNA) gene expression profiling require more attention. Addressing these issues is crucial for improving the accuracy and credibility of miRNA studies in biology.

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Last Updated: Jul 5, 2026

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

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Published on: August 3, 2011

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
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Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

Area of Science:

  • Molecular Biology
  • Genomics
  • Biochemistry

Background:

  • MicroRNA (miRNA) gene expression profiling is vital in plant and animal biology.
  • Limited research exists on technical challenges in miRNA profiling.
  • mRNA profiling literature offers insights but miRNA biochemistry presents unique considerations.

Purpose of the Study:

  • To highlight critical technical parameters and potential biases in miRNA gene expression profiling.
  • To emphasize the need for greater focus on methodological rigor in miRNA research.

Main Methods:

  • Review of existing literature on gene expression profiling (mRNA and miRNA).
  • Identification of unique biochemical and regulatory aspects of miRNAs.
  • Analysis of potential sources of systematic bias in miRNA studies.

Main Results:

  • miRNA processing, stability, and regulation have unique biochemical properties.
  • Pre-analytical variables and enzyme specificities can introduce bias.
  • Challenges exist in miRNA discovery and annotation.

Conclusions:

  • Greater attention to technical parameters is essential for miRNA profiling.
  • Improving methodological rigor will enhance the validity and reliability of miRNA studies.
  • Addressing these factors is key to bolstering the credibility of miRNA research.