Related Experiment Video
Updated: Jul 5, 2026
Isolation and Direct Neuronal Reprogramming of Mouse Astrocytes
Published on: July 7, 2022
Induction of neural stem cell-like cells (NSCLCs) from mouse astrocytes by Bmi1
Jai-Hee Moon1, Byung Sun Yoon, Bona Kim
1Laboratory of Cell Growth and Function Regulation, College of Life Sciences and Biotechnology, Korea University, 5-1, Anam-Dong, Sungbuk-ku, Seoul 136-701, Republic of Korea.
Abstract:
Recently, Bmi1 was shown to control the proliferation and self-renewal of neural stem cells (NSCs). In this study, we demonstrated the induction of NSC-like cells (NSCLCs) from mouse astrocytes by Bmi1 under NSC culture conditions. These NSCLCs exhibited the morphology and growth properties of NSCs, and expressed NSC marker genes, including nestin, CD133, and Sox2. In vitro differentiation of NSCLCs resulted in differentiated cell populations containing astrocytes, neurons, and oligodendrocytes. Following treatment with histone deacetylase inhibitors (trichostatin A and valproic acid), the potential of NSCLCs for proliferation, dedifferentiation, and self-renewal was significantly inhibited. Our data indicate that multipotent NSCLCs can be generated directly from astrocytes by the addition of Bmi1.
